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Updated: Mar 31, 2026

Isolating Brown Adipocytes from Murine Interscapular Brown Adipose Tissue for Gene and Protein Expression Analysis
Published on: March 12, 2021
A comparison of PGC-1α mRNA and protein expression in response to 1-week endurance training on alternate days or 4
Li-Ping Huang1, Min Yao1, Ya-Li Wang1
1a Department of Health and Exercise Science, Tianjin University of Sport, Tianjin 300381, China.
Abstract:
To understand the molecular mechanisms of adaptation to different training protocols, this study compared the effects of 4 sessions of 90 min treadmill exercise on alternate days or consecutive days in 1 week on messenger RNA (mRNA) and protein expression of proliferator-activated receptor-γ coactivator 1-α in rat gastrocnemius muscle. The mRNA significantly increased by 25.8-fold after alternate-day and 10.1-fold after consecutive-day training, while the protein showed no significant cumulative effect, 1.5-1.7-fold above baseline, in the 2 protocols.

