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Updated: Mar 31, 2026

Post Column Derivatization Using Reaction Flow High Performance Liquid Chromatography Columns
Published on: April 26, 2016
Current status and recent advantages in derivatization procedures in human doping control.
Ioanna Athanasiadou1,2, Polyxeni Kiousi3, Nasia Kioukia-Fougia3
1Laboratory of Biopharmaceutics & Pharmacokinetics, Faculty of Pharmacy, National & Kapodistrian University of Athens, Greece.
Derivatization enhances chromatographic separation and mass spectrometric detection in doping control analysis. This crucial sample preparation step broadens the detectable range of compounds for athlete drug testing.
Area of Science:
- Analytical Chemistry
- Forensic Science
Background:
- Sample preparation is critical in doping control analysis.
- Derivatization is a key technique for improving analyte detection.
Purpose of the Study:
- To highlight the importance of derivatization in doping control.
- To explain how derivatization enhances chromatographic and mass spectrometric analysis.
Main Methods:
- Review of derivatization applications in doping control.
- Discussion of its impact on metabolic studies and detection procedures.
Main Results:
- Derivatization significantly enhances separation and detection of analytes.
- It broadens the range of detectable compounds, even in LC-MS.
- It is vital from metabolic studies to confirmation of prohibited substances.
Conclusions:
- Derivatization is an essential technique in doping control analysis.
- It offers advanced analyte detection capabilities for identifying prohibited substances.
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