Related Experiment Videos
Nucleotides in yeast tRNAPhe required for the specific recognition by its cognate synthetase
J R Sampson1, A B DiRenzo, L S Behlen
1Department of Chemistry and Biochemistry, University of Colorado, Boulder 80309.
Science (New York, N.Y.)
|March 10, 1989
Abstract:
An analysis of the aminoacylation kinetics of unmodified yeast tRNAPhe mutants revealed that five single-stranded nucleotides are important for its recognition by yeast phenylalanyl-tRNA synthetase, provided they were positioned correctly in a properly folded tRNA structure. When four other tRNAs were changed to have these five nucleotides, they became near-normal substrates for the enzyme.