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Platelet Adhesion and Aggregation Under Flow using Microfluidic Flow Cells

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Measuring platelet function with platelet shape change, an early event in aggregation.

D O Beaumont¹, K W Whitten, R G Mock

¹Department of Biochemistry, Loma Linda University School of Medicine, CA 92350.

Thrombosis Research

|January 15, 1989

Summary

Platelet shape change, a sensitive indicator of activation, can be reliably measured using a novel spectrophotometric method. This technique offers a sensitive alternative to traditional platelet aggregation assays.

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Area of Science:

Hematology
Biophysics
Biochemistry

Background:

Platelet shape change is an early indicator of platelet activation, preceding aggregation.
Quantifying shape change offers a sensitive method for assessing platelet responsiveness to stimuli.

Purpose of the Study:

To develop and validate a spectrophotometric method for measuring platelet shape change in response to Platelet Activating Factor (PAF).
To correlate shape change measurements with other indicators of platelet activation.

Main Methods:

Utilized differences in light transmission (%T) through platelet suspensions to quantify shape changes.
Defined a Shape Change Parameter (SCP) and analyzed its dose-dependent relationship with PAF.
Correlated SCP with platelet morphology via phase contrast microscopy and performed linear regression analysis.

Main Results:

A novel Shape Change Parameter (SCP) was defined, showing a clear dose-response to PAF.
The SCP demonstrated high sensitivity, with dose-response curves similar for human and sheep platelets.
Linear regression revealed a strong correlation (R=0.990) between SCP and percent platelet activation, indicating a 1:1 correspondence.

Conclusions:

The developed SCP method provides a sensitive, reliable, and convenient alternative to platelet aggregation assays for measuring platelet activation.
This method accurately reflects platelet morphological changes in response to stimuli.