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Updated: Mar 31, 2026

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ScanLag: High-throughput Quantification of Colony Growth and Lag Time
Published on: July 15, 2014
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Quantitative Measurements of Type I and Type II Persisters Using ScanLag
Irit Levin-Reisman1, Nathalie Q Balaban2
1The Racah Institute of Physics, The Hebrew University of Jerusalem, Jerusalem, 91904, Israel.
Methods in Molecular Biology (Clifton, N.J.)
|October 16, 2015
Abstract:
The present method quantifies the number of slow-growing bacteria leading to antibiotic persistence in a clonal population. First, it enables discriminating between slow growers that are generated by exposure to a stress signal (Type I persisters) and slow growers that are continuously generated during exponential growth (Type II persisters). Second, the method enables determining the amount of slow growers in a culture.

