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Updated: Mar 31, 2026
High-throughput Assay to Phenotype Salmonella enterica Typhimurium Association, Invasion, and Replication in Macrophages
Published on: August 11, 2014
Analysis of Macrophage-Induced Salmonella Persisters
Robert A Fisher1, Angela M Cheverton1, Sophie Helaine2
1Section of Microbiology, Medical Research Council Centre for Molecular Bacteriology and Infection, Imperial College London, South Kensington Campus, Armstrong Road, London, SW7 2AZ, UK.
Abstract:
A small subpopulation of non-replicating, multidrug-tolerant bacteria is present within clonal populations of many bacterial species. Known as persisters, these bacteria are probably the cause of relapsing infections such as typhoid fever. Formation of non-growing Salmonella persisters is stimulated by macrophage phagocytosis. This chapter outlines methods to identify and study persisters resulting from interactions between bacterial pathogens and their hosts. We use their antibiotic tolerance for isolation and enumeration and developed a method to study the heterogeneity of growth within clonal populations through single-cell analysis.
