Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Cleavage map of linear mouse sarcoma virus DNA.

E Canaani, P Duesberg, D Dina

    Proceedings of the National Academy of Sciences of the United States of America
    |January 1, 1977
    PubMed
    Summary

    Researchers characterized Moloney murine sarcoma virus DNA, identifying linear, circular, and integrated forms. Restriction enzyme analysis revealed unique ends and a genome complexity of 3.4 x 10^6 daltons, suggesting redundant sequences at termini.

    Related Concept Videos

    You might also read

    Related Articles

    Articles linked to this work by shared authors, journal, and citation graph.

    Sort by
    Same author

    Induction of centrosome and chromosome aberrations by imatinib in vitro.

    Leukemia·2005
    Same author

    ALL-1/MLL1, a homologue of Drosophila TRITHORAX, modifies chromatin and is directly involved in infant acute leukaemia.

    British journal of cancer·2004
    Same author

    Expression profiles of acute lymphoblastic and myeloblastic leukemias with ALL-1 rearrangements.

    Proceedings of the National Academy of Sciences of the United States of America·2003
    Same author

    Trithorax and dCBP acting in a complex to maintain expression of a homeotic gene.

    Science (New York, N.Y.)·2001
    Same author

    Origin of multidrug resistance in cells with and without multidrug resistance genes: chromosome reassortments catalyzed by aneuploidy.

    Proceedings of the National Academy of Sciences of the United States of America·2001
    Same author

    Upregulation of Meis1 and HoxA9 in acute lymphocytic leukemias with the t(4 : 11) abnormality.

    Oncogene·2001

    Area of Science:

    • Molecular Biology
    • Virology
    • Genetics

    Background:

    • Moloney murine sarcoma virus (MMSV) is a retrovirus known to cause tumors in mice.
    • Understanding the structure and replication of viral DNA is crucial for developing antiviral therapies.
    • Previous studies have focused on viral RNA, but the proviral DNA forms require detailed characterization.

    Purpose of the Study:

    • To isolate and characterize the proviral DNA forms transcribed from Moloney murine sarcoma virus RNA.
    • To determine the structure, complexity, and end-specific sequences of the linear viral DNA.
    • To map the cleavage sites of restriction endonucleases on the linear viral DNA.

    Main Methods:

    • Isolation of proviral DNA from newly infected cells.
    • Separation and identification of different viral DNA forms (linear, fast-sedimenting, circular) using sedimentation analysis.
    • Restriction endonuclease digestion of linear viral DNA.
    • Electrophoretic analysis of DNA fragments.
    • Hybridization of DNA fragments with viral RNA and cDNA probes.

    Main Results:

    • Three forms of viral DNA were identified: linear (3.4 x 10^6 daltons), fast-sedimenting (integrated or concatemers), and covalently closed circles.
    • Linear viral DNA exhibited unique ends and a complexity of 3.4 x 10^6 daltons.
    • Specific restriction endonucleases (HindIII, Hae II, HincII) cleaved the linear DNA into distinct fragments, allowing for ordering.
    • Fragments from both ends of the linear DNA hybridized to the 3' end of viral RNA, indicating potential redundant sequences.

    Conclusions:

    • The study elucidated the structural organization of Moloney murine sarcoma virus proviral DNA.
    • Linear viral DNA possesses unique termini and a defined complexity, serving as a complete transcript of viral RNA.
    • The presence of redundant sequences at both termini of the viral DNA genome was suggested, impacting viral integration and replication.

    Related Experiment Videos