Class A and D Extended-Spectrum β-Lactamases in Imipenem Resistant Pseudomonas aeruginosa Isolated From Burn Patients

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Isolation and Identification of Waterborne Antibiotic-Resistant Bacteria and Molecular Characterization of their Antibiotic Resistance Genes
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Isolation and Identification of Waterborne Antibiotic-Resistant Bacteria and Molecular Characterization of their Antibiotic Resistance Genes

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Sanaz Pakbaten Toupkanlou1, Shahin Najar Peerayeh1, Rahim Pirhajati Mahabadi1

  • 1Department of Bacteriology, Faculty of Medical Sciences, Tarbiat Modares University, Tehran, IR Iran.

Jundishapur Journal of Microbiology
|October 16, 2015
PubMed
Abstract

Insights

Multidrug-resistant Pseudomonas aeruginosa, a major cause of burn wound infections, frequently exhibits resistance genes like bla PER, bla OXA-10, bla TEM, and bla SHV in Iran. This poses a significant threat to burn care.

Area of Science:

  • Medical Microbiology
  • Infectious Diseases
  • Antimicrobial Resistance

Background:

  • Pseudomonas aeruginosa is a critical pathogen in burn wound infections, leading to significant mortality.
  • Antibiotic resistance in P. aeruginosa complicates treatment and increases patient risk.

Purpose of the Study:

  • To determine the prevalence of Ambler class A and D beta-lactamases in P. aeruginosa from burn infections in Tehran, Iran.
  • To investigate the genetic basis of resistance in these isolates.

Main Methods:

  • Bacteriological samples were collected from burn patients with clinical signs of infection.
  • Fifty P. aeruginosa isolates were identified based on phenotypic characteristics and confirmed by PCR.
  • Antibiotic susceptibility testing and phenotypic screening for beta-lactamases were performed, followed by PCR for specific resistance genes (bla PER, bla OXA-10, bla TEM, bla SHV, bla KPC, bla GES).

Main Results:

  • 88% of P. aeruginosa isolates were multidrug-resistant.
  • Prevalence of specific beta-lactamase genes: bla PER (14%), bla OXA-10 (36%), bla TEM (36%), and bla SHV (36%).
  • No isolates carried bla KPC or bla GES genes.

Conclusions:

  • High levels of multidrug resistance and the presence of PER, OXA-10, SHV, and TEM beta-lactamases are prevalent in imipenem-resistant P. aeruginosa from Iranian burn patients.
  • This resistance pattern represents a substantial threat to burn centers in Iran.
  • Urgent strategies are needed to combat the spread of antibiotic resistance in P. aeruginosa.
Keywords:
ImipenemPseudomonas aeruginosablaPER, blaOXA-10, blaTEM, blaSHV

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