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Updated: Jul 19, 2026
In Situ Detection of Bacteria within Paraffin-embedded Tissues Using a Digoxin-labeled DNA Probe Targeting 16S rRNA
Published on: May 21, 2015
Detection of Epstein-Barr virus in different sources of materials from patients with oral lichen planus: a
Rúbia da Rocha Vieira1, Lígia Lavezo Ferreira1, Éder Ricardo Biasoli1
1Oral Oncology Center and Department of Pathology and Clinical Propaedeutics, Araçatuba School of Dentistry, Araçatuba, São Paulo, Brazil.
Aims:
To detect the presence of Epstein-Barr virus (EBV) DNA in different sources of materials from a matched group of patients with oral lichen planus (OLP) and a group of people without OLP lesions, and to correlate the presence of virus with epidemiological variables of the groups studied.
Methods:
Fresh tissue samples, saliva, exfoliated cells and plasma of 24 patients with OLP lesions (cases) and 17 patients without OLP lesions (controls) were collected. EBV was detected by nested PCR.
Results:
Viral positivity was obtained in 62.5% of tissue samples; in 70.8% of exfoliated cell samples; in 33.3% of blood plasma samples and in 75% of saliva samples in the cases; and in 35.3% of tissue samples; 82.4% of exfoliated cell samples; in 47.1% of blood plasma samples and in 64.7% of saliva samples in the controls. There was a predominance of women in both groups. Variables not atrophic-erosive were most affected by EBV.
Conclusions:
No relationship between EBV and OLP was found. However, all sources tested in this study were considered suitable for the detection of viruses.
