Comparing Viability of Periodontal Ligament Stem Cells Isolated From Erupted and Impacted Tooth Root
Mohsen Dalband1, Iraj Amiri, Ali Reza Soltanian
1*Department of Oral and Maxillofacial Surgery, Dental Faculty, Shahid Beheshti University of Medical Science, Tehran †Department of Anatomy and Embryology, Medical Faculty, Hamadan University of Medical Sciences ‡Department of Biostatistics and Epidemiology, Research Center for Modeling of Noncommunicable Diseases, Public Health Faculty, Hamadan University of Medical Sciences, Hamadan §Department of Oral and Maxillofacial Surgery, Maxillofacial Diseases Research Center, Dental Faculty, Mashhad University of Medical Science, Mashhad ||Department of Oral and Maxillofacial Surgery, Dental Faculty, Kurdestan University of Medical Science, Sanandaj ¶Private Practice, Hamadan, Iran.
Purpose:
The aim of the study was to compare the viability of periodontal ligament-derived stem/progenitor cells (PDLSCs) from 2 different sources.
Materials And Methods:
Periodontal ligament (PDL) tissue was obtained from 20 surgically extracted human third molars and 20 healthy premolars extracted for orthodontic reasons. Periodontal ligament-derived stem/progenitor cells were isolated from 2 different PDL tissue sources and characterized by colony forming unit assay, cell surface marker characterizations, and their osteogenic differentiation potential. To determine cell viability within 2 groups, the colorimetric 3-(4, 5-dimethylthiazol-2-yl)-2, 5-diphenyl tetrazolium bromide (MTT) metabolic activity assay was used. Data were statistically analyzed using independent t-test by SPSS 16 software (SPSS Inc, Chicago, IL).
Results:
According to the MTT assay, the mean viability rate ± standard deviation of PDLSCs in the impacted third molar sample cells was 0.355 ± 0.411 and for erupted premolar sample cells was 0.331 ± 0.556. Based on One-Sample Kolmogorov-Smirnov test, P value for impacted and erupted teeth was 0.954 and 0.863, respectively. No statistical difference was seen between 2 groups. (P value > 0.05) CONCLUSIONS: Our results demonstrated that if surgical aseptic technique is a method employed to maintain asepsis, PDLSCs obtained from impacted and erupted tooth root would have the same viability rate.
