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    Area of Science:

    • Biomedical optics
    • Medical imaging technology
    • Blood flow quantification

    Background:

    • Laser speckle contrast imaging (LSCI) is a valuable tool for assessing blood flow.
    • Traditional LSCI methods can be limited by acquisition time and light level regulation.
    • High frame rate imaging offers potential for improved LSCI performance.

    Purpose of the Study:

    • To develop and evaluate a multi-exposure laser speckle contrast imaging (LSCI) system.
    • To compare the performance of multi-exposure LSCI with laser Doppler imaging (LDI).
    • To investigate the linearity of multi-exposure LSCI and LDI responses to blood flow velocity.

    Main Methods:

    • Implementation of multi-exposure LSCI using a high frame rate CMOS sensor and FPGA processing.
    • Simulation of different exposure times by accumulating short exposures.
    • Direct comparison of multi-exposure LSCI and LDI using identical experimental data from a rotating diffuser.

    Main Results:

    • The developed system allows for flexible simulation of exposure times without light level adjustments.
    • Both multi-exposure LSCI and LDI demonstrated a linear response to changes in velocity.
    • Single-exposure LSCI requires specific models for accurate velocity response correction.

    Conclusions:

    • Multi-exposure LSCI implemented on a high frame rate system provides a robust method for blood flow imaging.
    • The system enables direct comparison with LDI, validating its performance.
    • Multi-exposure LSCI offers a linear velocity response, advantageous over single-exposure LSCI without correction.