Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Phase Contrast and Differential Interference Contrast Microscopy01:26

Phase Contrast and Differential Interference Contrast Microscopy

15.2K
Phase-Contrast Microscopes
In-phase-contrast microscopes, interference between light directly passing through a cell and light refracted by cellular components is used to create high-contrast, high-resolution images without staining. It is the oldest and simplest type of microscope that creates an image by altering the wavelengths of light rays passing through the specimen. Altered wavelength paths are created using an annular stop in the condenser. The annular stop produces a hollow cone of...
15.2K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Nano helical cholesteric liquid crystals exhibit long term bistability for energy saving smart windows.

Nanoscale advances·2026
Same author

Fast polarization independent tunable liquid crystal Solc filter using Sagnac configuration.

Optics letters·2025
Same author

Fast tunable metamaterial liquid crystal achromatic waveplate.

Nanophotonics (Berlin, Germany)·2024
Same author

3D nanoplasmonic structure for ultrahigh enhanced SERS with less variability, polarization independence, and multimodal sensing applied to picric acid detection.

Nanoscale advances·2024
Same author

Identification of spermatogenesis in individual seminiferous tubules and testicular tissue of adult normal and busulfan-treated mice employing Raman spectroscopy and principal component analysis.

Spectrochimica acta. Part A, Molecular and biomolecular spectroscopy·2024
Same author

Compressive hyperspectral microscopy for cancer detection.

Journal of biomedical optics·2023

Related Experiment Video

Updated: Mar 31, 2026

A Multimodal Wide-Field Fourier-Transform Raman Microscope
06:48

A Multimodal Wide-Field Fourier-Transform Raman Microscope

Published on: December 30, 2025

750

High-speed 3D imaging using two-wavelength parallel-phase-shift interferometry.

Avner Safrani, Ibrahim Abdulhalim

    Optics Letters
    |October 16, 2015
    PubMed
    Summary

    High-speed 3D imaging uses two-wavelength interferometry for precise measurements. This advanced technique achieves nanometer accuracy for high step profiles, ideal for semiconductor applications.

    Area of Science:

    • Optical Metrology
    • Nanotechnology
    • Semiconductor Manufacturing

    Background:

    • Accurate 3D surface profiling is critical for microelectronics fabrication.
    • Existing high-speed imaging techniques often lack the required vertical accuracy for complex structures.

    Purpose of the Study:

    • To present a high-speed, high-accuracy three-dimensional (3D) imaging technique.
    • To enable video-rate imaging of high step profiles with nanometer precision.

    Main Methods:

    • Utilized two-wavelength parallel-phase-shift interferometry.
    • Employed a polarization-based Linnik interferometer with three phase-masked CCD cameras.
    • Incorporated two quasi-monochromatic modulated light sources for phase unwrapping.

    More Related Videos

    Three-dimensional Super Resolution Microscopy of F-actin Filaments by Interferometric PhotoActivated Localization Microscopy iPALM
    11:57

    Three-dimensional Super Resolution Microscopy of F-actin Filaments by Interferometric PhotoActivated Localization Microscopy iPALM

    Published on: December 1, 2016

    11.3K
    High-resolution, High-speed, Three-dimensional Video Imaging with Digital Fringe Projection Techniques
    11:34

    High-resolution, High-speed, Three-dimensional Video Imaging with Digital Fringe Projection Techniques

    Published on: December 3, 2013

    16.2K

    Related Experiment Videos

    Last Updated: Mar 31, 2026

    A Multimodal Wide-Field Fourier-Transform Raman Microscope
    06:48

    A Multimodal Wide-Field Fourier-Transform Raman Microscope

    Published on: December 30, 2025

    750
    Three-dimensional Super Resolution Microscopy of F-actin Filaments by Interferometric PhotoActivated Localization Microscopy iPALM
    11:57

    Three-dimensional Super Resolution Microscopy of F-actin Filaments by Interferometric PhotoActivated Localization Microscopy iPALM

    Published on: December 1, 2016

    11.3K
    High-resolution, High-speed, Three-dimensional Video Imaging with Digital Fringe Projection Techniques
    11:34

    High-resolution, High-speed, Three-dimensional Video Imaging with Digital Fringe Projection Techniques

    Published on: December 3, 2013

    16.2K

    Main Results:

    • Successfully imaged step profiles up to 3.7 μm with ±2 nm accuracy and repeatability.
    • Demonstrated video-rate imaging capabilities.
    • Validated the technique on a certified very large scale integration (VLSI) step standard and semiconductor integrated chips with copper micro pillars.

    Conclusions:

    • The developed two-wavelength interferometry technique offers high-speed and high-accuracy 3D imaging.
    • This method is suitable for critical dimension metrology in semiconductor manufacturing.
    • The technique provides a robust solution for profiling complex microelectronic structures.