Quantitative bioanalytical validation of fosfomycin in human whole blood with volumetric absorptive microsampling

Fosfomycin is an antibiotic of considerable interest for the treatment of infection by multidrug-resistant bacteria. Translating microsampling techniques into clinical PK studies may provide effective dosing information to improve patient outcomes and minimize the potential development of resistance.

Accuracy and precision results were within ±15%; the method was validated across the range of 5-2000 µg/ml of fosfomycin using volumetric absorptive microsampling (VAMS) devices.

The VAMS techniques provide acceptable validation data as assessed for lower limit of quantification, linearity, intra- and interday precision and accuracy, selectivity and matrix effects. Results from the recovery and stability studies suggest challenges remain for the analysis of fosfomycin in whole blood using VAMS.