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Updated: Mar 31, 2026

In vitro Digestion of Emulsions in a Single Droplet via Multi Subphase Exchange of Simulated Gastrointestinal Fluids
Published on: November 18, 2022
Dynamic modeling of in vitro lipid digestion: individual fatty acid release and bioaccessibility kinetics
T M Giang1, S Gaucel1, P Brestaz2
1INRA, UMR782 Génie et Microbiologie des Procédés Alimentaires, F-78850 Thiverval Grignon, France; AgroParisTech, UMR782 Génie et Microbiologie des Procédés Alimentaires, F-78850 Thiverval Grignon, France.
Abstract:
The aim of this study was to gain knowledge about the role of triacylglycerol (TAG) composition in fatty acids (FA) of o/w emulsions on both the pancreatic lipolysis kinetics and the bioaccessibility of released products (i.e. contained within the bile salt micellar phase). A mathematical model was developed and its predictions were compared to a set of experimental data obtained during an in vitro digestion of a whey protein stabilized emulsion. Modeling results show that FA residues of TAG were hydrolyzed at specific rates, inducing different bioaccessibility kinetics. The estimated lipolysis rate constants of the studied FA (C8:0, C10:0≫C18:1 n-9≫C12:0>C14:0>C16:0≈C16:1 n-7>C22:6 n-3) were in close agreement with the available literature on the substrate specificity of pancreatic lipase. Results also suggest that lipolysis products are very rapidly solubilized in the bile salt mixed micelles with no fractionation according to the FA carbon chain.
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