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Related Concept Videos

Site-Targeted Drug Delivery Systems: Polymeric Carriers01:24

Site-Targeted Drug Delivery Systems: Polymeric Carriers

109
Polymeric carriers enhance targeted drug delivery by increasing efficacy while minimizing off-target effects. These carriers comprise a biodegradable polymeric backbone integrated with functional elements that enable targeting, improve physicochemical properties, and regulate drug release.Targeting MechanismsThe targeting ability of polymeric carriers is mediated by a homing device, which is a molecular recognition component designed to selectively bind to specific tissues or cells. Monoclonal...
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Modified-Release Drug Delivery Systems: Site-Targeted01:24

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Site-targeted drug delivery systems enhance therapeutic efficacy while minimizing systemic toxicity and treatment costs. Unlike conventional methods, these systems ensure precise drug delivery, improving bioavailability and reducing side effects. Targeted drug delivery is classified into three levels. First-order targeting directs drugs to the capillary beds of specific organs or tissues. Second-order targets specific cell types, such as tumor cells, using receptor-mediated interactions.
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Related Experiment Video

Updated: Mar 31, 2026

Preparation of Neutrally-charged, pH-responsive Polymeric Nanoparticles for Cytosolic siRNA Delivery
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Liver-Targeted SiRNA Delivery Using Biodegradable Poly(amide) Polymer Conjugates.

Stephanie E Barrett1, Erin N Guidry2

  • 1Pharmaceutical Sciences and Clinical Supply, Merck Research Laboratories, 770 Sumneytown Pike, 4, West Point, PA, 19486, USA. stephanie_barrett@merck.com.

Methods in Molecular Biology (Clifton, N.J.)
|October 17, 2015
PubMed
Summary

Developing novel polymer conjugate formulations is crucial for clinical RNA delivery. This study presents a robust method for screening poly(amine)-based polymers for small interfering RNA (siRNA) delivery, including synthesis and characterization.

Keywords:
Biodegradable polymersPolyamidePolymer conjugatesSiRNA deliverySiRNA protocolsTargeted delivery

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Area of Science:

  • Biomaterials Science
  • Polymer Chemistry
  • Drug Delivery Systems

Background:

  • Clinical translation of RNA therapeutics necessitates efficient and safe delivery systems.
  • Polymer conjugates offer a promising platform for targeted RNA delivery.
  • Existing methods for screening siRNA delivery polymers are often complex and time-consuming.

Purpose of the Study:

  • To establish a facile and robust protocol for screening poly(amine)-based polymers for small interfering RNA (siRNA) delivery.
  • To detail the synthesis and characterization of novel poly(amide) polymers and their targeted siRNA conjugates.
  • To provide a reproducible method for evaluating biodegradable poly(peptide) and polymer conjugate performance.

Main Methods:

  • Controlled polymerization techniques for synthesizing poly(amide) polymers.
  • Preparation of targeted siRNA-polymer conjugates.
  • Characterization of biodegradable poly(peptides) and resulting polymer conjugates.

Main Results:

  • Successful synthesis of poly(amide) polymers using controlled polymerization.
  • Preparation of targeted siRNA-polymer conjugates.
  • Detailed characterization data for the synthesized polymers and conjugates.

Conclusions:

  • The described protocols offer a robust and facile approach for screening poly(amine)-based polymer systems for siRNA delivery.
  • This work facilitates the development of optimized polymer conjugate formulations for clinical RNA delivery.
  • The methodology supports the advancement of biodegradable poly(peptide) and polymer conjugate research for therapeutic applications.