Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Genomic Medicine Sweden: Advancing precision medicine at the national level.

Journal of internal medicine·2026
Same author

Carbon dot-based polyplexes with cell penetration peptides for gene transfection.

RSC advances·2025
Same author

A Physiologically Based Pharmacokinetic (PBPK) Study to Assess the Adjuvanticity of Three Peptides in an Oral Vaccine.

Pharmaceutics·2024
Same author

Cell-Penetrating Peptides: Promising Therapeutics and Drug-Delivery Systems for Neurodegenerative Diseases.

Molecular pharmaceutics·2024
Same author

A Method for Using Cell-Penetrating Peptides for Loading Plasmid DNA into Secreted Extracellular Vesicles.

Biomolecules·2023
Same author

Aggregation Limiting Cell-Penetrating Peptides Derived from Protein Signal Sequences.

International journal of molecular sciences·2023

Related Experiment Video

Updated: Mar 31, 2026

Author Spotlight: Optimization of Ultrashort Peptide Matrices for Colorectal Cancer Organoids
10:23

Author Spotlight: Optimization of Ultrashort Peptide Matrices for Colorectal Cancer Organoids

Published on: May 3, 2024

1.6K

PepFect6 Mediated SiRNA Delivery into Organotypic Cultures.

Suvarna Dash-Wagh1, Ülo Langel2, Mats Ulfendahl3

  • 1Department of Neuroscience, Karolinska Institutet, Retzeus väg 8, Stockholm, 17177, Sweden. Suvarna.dash-wagh@ki.se.

Methods in Molecular Biology (Clifton, N.J.)
|October 17, 2015
PubMed
Summary

Gene silencing using small interfering RNA (siRNA) faces delivery challenges. A new PepFect6 (PF6) peptide system effectively delivers siRNA into inner ear cells, enabling robust RNA interference responses for potential therapeutic applications.

Keywords:
Cell-penetrating peptides (CPPs )CochleaFluorescence recovery after photobleaching (FRAP)Inner earOrganotypic culturesPepFect6 (PF6 )SiRNA

More Related Videos

Author Spotlight: Generation and Manipulation of Rat Intestinal Organoids
09:49

Author Spotlight: Generation and Manipulation of Rat Intestinal Organoids

Published on: June 23, 2023

5.9K
High efficiency, Site-specific Transfection of Adherent Cells with siRNA Using Microelectrode Arrays MEA
09:14

High efficiency, Site-specific Transfection of Adherent Cells with siRNA Using Microelectrode Arrays MEA

Published on: September 13, 2012

14.0K

Related Experiment Videos

Last Updated: Mar 31, 2026

Author Spotlight: Optimization of Ultrashort Peptide Matrices for Colorectal Cancer Organoids
10:23

Author Spotlight: Optimization of Ultrashort Peptide Matrices for Colorectal Cancer Organoids

Published on: May 3, 2024

1.6K
Author Spotlight: Generation and Manipulation of Rat Intestinal Organoids
09:49

Author Spotlight: Generation and Manipulation of Rat Intestinal Organoids

Published on: June 23, 2023

5.9K
High efficiency, Site-specific Transfection of Adherent Cells with siRNA Using Microelectrode Arrays MEA
09:14

High efficiency, Site-specific Transfection of Adherent Cells with siRNA Using Microelectrode Arrays MEA

Published on: September 13, 2012

14.0K

Area of Science:

  • Biotechnology
  • Molecular Biology
  • Gene Therapy

Background:

  • Gene silencing via small interfering RNA (siRNA) is a promising therapeutic strategy.
  • Challenges include rapid RNA degradation and poor cellular uptake of naked siRNA.
  • Cell-penetrating peptides (CPPs) offer a non-cytotoxic solution for intracellular siRNA delivery.

Purpose of the Study:

  • To describe a protocol for siRNA delivery into organotypic cochlear cultures using PepFect6 (PF6) nanoparticle technology.
  • To optimize PF6-siRNA complex preparation and transfection for inner ear applications.
  • To analyze the efficacy of PF6-siRNA mediated RNA interference (RNAi) in the cochlea.

Main Methods:

  • Utilized PepFect6 (PF6), an amphipathic stearyl-TP10 peptide with a pH-titratable moiety for endosomal release.
  • Formed stable, non-covalent PF6-siRNA complexes.
  • Delivered complexes into organotypic cochlear cultures.

Main Results:

  • PF6 facilitates efficient siRNA internalization and rapid endosomal escape.
  • PF6-siRNA complexes induce robust RNA interference (RNAi) responses in cochlear cells.
  • The protocol highlights critical steps for successful PF6-siRNA delivery and efficacy analysis.

Conclusions:

  • PF6-nanoparticle technology provides an effective method for siRNA delivery to inner ear organotypic cultures.
  • This approach overcomes limitations of naked siRNA, enabling potent gene silencing.
  • The described protocol is valuable for studying inner ear disorders and developing siRNA-based therapeutics.