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Long non-coding RNAs as novel expression signatures modulate DNA damage and repair in cadmium toxicology

Zhiheng Zhou1, Haibai Liu1, Caixia Wang2

  • 1School of Public Health, Guangzhou Medical University, Guangzhou 510182, People's Republic of China.

Scientific Reports
|October 17, 2015
PubMed

Insights

Long non-coding RNAs (lncRNAs) show altered expression in cadmium toxicity. The lncRNA ENST00000414355 impacts DNA damage and repair, potentially serving as a cadmium toxicity biomarker.

Area of Science:

  • Environmental Toxicology
  • Molecular Biology
  • Epigenetics

Background:

  • Long non-coding RNAs (lncRNAs) are increasingly recognized for their roles in cellular processes.
  • Cadmium (Cd) exposure is a significant environmental health concern linked to various toxicities.
  • The specific involvement of lncRNAs in cadmium-induced DNA damage and repair remains underexplored.

Purpose of the Study:

  • To investigate the role of lncRNAs in modulating DNA damage and repair pathways under cadmium toxicity.
  • To identify specific lncRNAs that exhibit altered expression profiles in response to cadmium exposure.
  • To evaluate the potential of lncRNAs as biomarkers for cadmium toxicity.

Main Methods:

  • Comparison of lncRNA expression profiles in cadmium-induced 16HBE cells versus control cells.
  • siRNA-mediated knockdown of the identified lncRNA ENST00000414355.
  • Analysis of DNA damage and repair gene expression following lncRNA knockdown.
  • Assessment of ENST00000414355 expression in cadmium-exposed rats and human workers.

Main Results:

  • Aberrant lncRNA expression profiles were observed in cadmium-treated cells.
  • Knockdown of ENST00000414355 inhibited DNA-damaged cell growth and altered DNA damage/repair gene expression.
  • Cadmium exposure increased ENST00000414355 expression in rat lungs in a dose-dependent manner.
  • A positive correlation was found between ENST00000414355 expression and cadmium levels in rats and human workers.

Conclusions:

  • lncRNAs are aberrantly expressed in cadmium-treated cells, suggesting their involvement in cadmium toxicity.
  • lncRNA-ENST00000414355 plays a role in regulating DNA damage and repair.
  • ENST00000414355 may serve as a novel biomarker for cadmium toxicity, potentially through epigenetic mechanisms.

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