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Related Experiment Video

Updated: Mar 31, 2026

A Simple Microaspiration Technique for Isolating Somatic Cells from Cryopreserved Equine Semen as Nuclear Donors for Cloning
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Handmade cloning: recent advances, potential and pitfalls.

Geetika Verma1, J S Arora1, R S Sethi1

  • 1School of Animal Biotechnology, Post Graduate Institute of Veterinary Education and Research, Guru Angad Dev Veterinary and Animal Sciences University, Ludhiana, 141004 Punjab India.

Journal of Animal Science and Biotechnology
|October 17, 2015
PubMed
Summary

Handmade cloning (HMC), a simpler somatic cell nuclear transfer (SCNT) method, eliminates the need for micromanipulators and skilled expertise. This technique offers comparable or higher birth rates, reduced costs, and applications in species conservation and therapeutic development.

Keywords:
Handmade cloningMicromanipulatorSomatic cell nuclear transferTransgenicZona free cloning

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Area of Science:

  • Reproductive Biology
  • Developmental Biology
  • Animal Biotechnology

Background:

  • Somatic cell nuclear transfer (SCNT) is a key technique in reproductive biology.
  • Traditional cloning (TC) relies on expensive micromanipulators and specialized expertise.
  • There is a need for more accessible and cost-effective cloning methods.

Purpose of the Study:

  • To review the advancements and applications of Handmade Cloning (HMC).
  • To highlight factors influencing HMC efficiency.
  • To discuss the potential and limitations of HMC.

Main Methods:

  • Review of existing literature on Handmade Cloning (HMC).
  • Analysis of modifications aimed at improving HMC efficiency.
  • Exploration of HMC's applicability in various contexts.

Main Results:

  • HMC is a micromanipulator-free SCNT alternative.
  • HMC offers comparable or superior birth rates to TC.
  • HMC significantly reduces costs and enables cryopreservation.

Conclusions:

  • HMC represents a significant advancement in embryology, making cloning more accessible.
  • HMC has broad applications including intraspecies and interspecies nuclear transfer (iSCNT) for conservation.
  • HMC facilitates automation for producing transgenic animals for therapeutic purposes, benefiting human health.