A spectrometric method for hydrogen peroxide concentration measurement with a reusable and cost-efficient sensor

Area of Science:

• Analytical Chemistry
• Biomedical Engineering

Background:

• Hydrogen peroxide (H₂O₂) is a reactive oxygen species with significant implications in biological and chemical processes.
• Accurate and cost-effective H₂O₂ detection methods are crucial for various applications, including diagnostics and environmental monitoring.

Purpose of the Study:

• To develop and validate a low-cost spectrometric sensor for the quantitative analysis of hydrogen peroxide in liquid samples.
• To assess the sensor's performance characteristics, including measurement range, linearity, sensitivity, resolution, and reusability.

Main Methods:

• A spectrometric method was employed using a sensor based on immobilized peroxidase enzyme on a glass substrate.
• The enzymatic reaction catalyzed by peroxidase converts H₂O₂ into water and oxygen.
• A reagent (4-amino-phenazone and phenol) reacts with oxygen to produce a colored product with distinct absorption peaks at 510 nm and 450 nm, enabling quantitative analysis.

Main Results:

• The sensor demonstrated high linearity for H₂O₂ concentrations ranging from 5 × 10⁻⁵% to 1 × 10⁻³% (0.5 ppm to 10 ppm).
• Achieved a sensitivity of 41,400 (photon count/%) and a resolution of 3.49 × 10⁻⁵% (0.35 ppm).
• Exhibited a rapid response time of less than 3 minutes and maintained performance over 10 reuse applications.

Conclusions:

• The developed low-cost spectrometric sensor provides a reliable and efficient method for hydrogen peroxide quantification in liquids.
• The sensor's performance metrics indicate its suitability for applications requiring sensitive and accurate H₂O₂ measurements.
• This technology offers a cost-effective alternative for H₂O₂ detection in various scientific and industrial fields.