Related Experiment Video
Updated: Mar 31, 2026

A Luciferase-fluorescent Reporter Influenza Virus for Live Imaging and Quantification of Viral Infection
Published on: August 14, 2019
Multi-Modal Imaging with a Toolbox of Influenza A Reporter Viruses
Vy Tran1,2, Daniel S Poole3, Justin J Jeffery4
1Medical Microbiology and Immunology, University of Wisconsin Madison, Madison, WI 53706, USA. vgtran@wisc.edu.
Abstract:
Reporter viruses are useful probes for studying multiple stages of the viral life cycle. Here we describe an expanded toolbox of fluorescent and bioluminescent influenza A reporter viruses. The enhanced utility of these tools enabled kinetic studies of viral attachment, infection, and co-infection. Multi-modal bioluminescence and positron emission tomography-computed tomography (PET/CT) imaging of infected animals revealed that antiviral treatment reduced viral load, dissemination, and inflammation. These new technologies and applications will dramatically accelerate in vitro and in vivo influenza virus studies.
More Related Videos
Related Concept Videos
Leaky Scanning
Influenza

