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Related Experiment Videos

Islet function after storage at -2 degrees C and -196 degrees C.

Y Hara1, H Taniguchi, K Ishihara

  • 1Second Department of Internal Medicine, Kobe University School of Medicine, Japan.

Diabetes Research and Clinical Practice
|February 15, 1989
PubMed
Summary
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Preserving pancreatic islets at -2 degrees C with dimethyl sulfoxide shows promise for type 1 diabetes treatment. This method maintained islet function and effectively lowered blood glucose in diabetic rats post-transplantation.

Area of Science:

  • Endocrinology
  • Transplantation Biology
  • Cryobiology

Background:

  • Type 1 diabetes treatment via islet transplantation necessitates effective islet preservation.
  • Ice formation during cryopreservation causes significant damage, hindering successful transplantation.
  • Developing satisfactory methods for islet storage remains a critical challenge.

Purpose of the Study:

  • To compare the functional integrity of rat islets after frozen storage at -2°C and -196°C.
  • To evaluate the efficacy of islet transplantation using preserved islets in a diabetic rat model.

Main Methods:

  • Rat islets were stored for 7 days at -2°C (Group A) and -196°C (Group B) in 10% dimethyl sulfoxide.
  • Islet function was assessed by measuring insulin release and 3H-leucine uptake.

Related Experiment Videos

  • Preserved islets were transplanted into streptozotocin-induced diabetic rats, with fasting plasma glucose monitored weekly.

    • Main Results:

    • Islets stored at -2°C (Group A) exhibited insulin release comparable to non-cooled controls (Group C).
    • Islets stored at -196°C (Group B) showed significantly lower insulin release.
    • Both preservation methods led to similar reductions in fasting plasma glucose in transplanted diabetic rats, with better islet recovery from Group A.

    Conclusions:

    • Preservation of islets at -2°C using dimethyl sulfoxide is a viable method for maintaining islet function and achieving therapeutic outcomes in type 1 diabetes.
    • This method offers a practical alternative to ultra-low temperature storage, showing potential for clinical application.