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Activation of farnesoid X receptor downregulates monocyte chemoattractant protein-1 in murine macrophage
Liangpeng Li1, Qian Zhang1, Jiahe Peng1
1Department of Biochemistry and Molecular Biology, College of Basic Medical Sciences, Third Military Medical University, 400038, Chongqing, China.
Abstract:
Farnesoid X receptor (FXR) is a member of the nuclear receptor superfamily, which plays important roles in bile acids/lipid homeostasis and inflammation. Monocyte chemoattractant protein-1 (MCP-1) contributes to macrophage infiltration into body tissues during inflammation. Here we investigated whether FXR can regulate MCP-1 expression in murine macrophage. FXR activation down regulate MCP-1 mRNA and protein levels in ANA-1 and Raw264.7 cells. Luciferase reporter assay, Gel shift and Chromatin immunoprecipitation assays have revealed that the activated FXR bind to the FXR element located in -738 bp ∼ -723 bp in MCP-1 promoter. These results suggested that FXR may serve as a novel target for regulating MCP-1 levels for the inflammation related diseases therapies.
