Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

What is Genetic Engineering?00:49

What is Genetic Engineering?

81.3K
Overview
81.3K
Recombinant DNA01:09

Recombinant DNA

104.8K
Overview
104.8K
Reporter Genes02:11

Reporter Genes

13.8K
Reporter genes are a type of protein-coding gene that are often tagged to a gene of interest. Once inside a target cell, reporter genes usually produce visually identifiable characteristics like fluorescence and luminescence when expressed along with the gene of interest. Thus, reporter genes “report” the presence or absence of genes of interest in an organism, determine the gene expression pattern, or track the physical location of a DNA segment or protein in the cell.
13.8K
Synthetic Biology02:55

Synthetic Biology

5.8K
Synthetic biology is an interdisciplinary science that involves using principles from disciplines such as engineering, molecular biology, cell biology, and systems biology. It involves remodeling existing organisms from nature or constructing completely new synthetic organisms for applications such as protein or enzyme production, bioremediation, value-added macromolecule production, and the addition of desirable traits to crops, to name a few.
Golden rice
Golden rice is a genetically modified...
5.8K
Bioreactor Controls-III01:22

Bioreactor Controls-III

51
Strain improvement is a foundational strategy in industrial microbiology aimed at maximizing microbial productivity, particularly because natural isolates typically yield commercially valuable products in very low concentrations. Although optimizing the culture medium and environmental conditions can improve yields, these adjustments are inherently limited by the organism’s genetic potential. As a result, the focus shifts toward genetic modifications to enhance biosynthetic capacity. The...
51
Experimental RNAi02:15

Experimental RNAi

8.3K
RNA interference (RNAi) is a cellular mechanism that inhibits gene expression by suppressing its transcription or activating the RNA degradation process. The mechanism was discovered by Andrew Fire and Craig Mello in 1998 in plants. Today, it is observed in almost all eukaryotes, including protozoa, flies, nematodes, insects, parasites, and mammals. This precise cellular mechanism of gene silencing has been developed into a technique that provides an efficient way to identify and determine the...
8.3K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

A comprehensive dataset of 32 million pentapeptide structures for high-throughput virtual screening.

Scientific data·2026
Same author

Development of an isothermal point of care method for detection of human papilloma virus (HPV).

Aspects of molecular medicine·2026
Same author

A two-component relay-switch biosensor enables precise and quantitative detection of the B. subtilis quorum peptide PhrF: Implications in probing the microbiome.

Biosensors & bioelectronics·2026
Same author

Targeting emerging respiratory pathogens with clamp peptides: Broad-spectrum inhibition of viral entry in 3D human lung models.

Molecular therapy : the journal of the American Society of Gene Therapy·2026
Same author

Health, safety training, and exposure reduction practices among U. S. fire investigators: a cross-sectional study.

Frontiers in public health·2026
Same author

Vaginal polycyclic aromatic hydrocarbons (PAHs), HPV infection, and vaginal microbiome in firefighters: A cross-sectional study.

Journal of hazardous materials·2026

Related Experiment Video

Updated: Mar 31, 2026

Forward Genetic Screen Using Transgenic Calcium Reporter Aequorin to Identify Novel Targets in Calcium Signaling
08:46

Forward Genetic Screen Using Transgenic Calcium Reporter Aequorin to Identify Novel Targets in Calcium Signaling

Published on: August 1, 2020

8.8K

Enabling Aequorin for Biotechnology Applications Through Genetic Engineering.

Kristen Grinstead1, Smita Joel1, Jean-Marc Zingg1

  • 1Department of Biochemistry and Molecular Biology, Miller School of Medicine, University of Miami, Miami, FL, 33136, USA.

Advances in Biochemical Engineering/Biotechnology
|October 18, 2015
PubMed
Summary

Engineered bioluminescent proteins like aequorin offer sensitive detection without external light. These molecular reporters are valuable for various biological sensing applications, including calcium detection and in vivo imaging.

Keywords:
AequorinBioluminescenceCalciumELISAImagingMutagenesisWhole-cell assays

More Related Videos

In Vivo Functional Brain Imaging Approach Based on Bioluminescent Calcium Indicator GFP-aequorin
12:15

In Vivo Functional Brain Imaging Approach Based on Bioluminescent Calcium Indicator GFP-aequorin

Published on: January 8, 2016

13.0K
In Vitro Selection of Engineered Transcriptional Repressors for Targeted Epigenetic Silencing
10:44

In Vitro Selection of Engineered Transcriptional Repressors for Targeted Epigenetic Silencing

Published on: May 5, 2023

2.1K

Related Experiment Videos

Last Updated: Mar 31, 2026

Forward Genetic Screen Using Transgenic Calcium Reporter Aequorin to Identify Novel Targets in Calcium Signaling
08:46

Forward Genetic Screen Using Transgenic Calcium Reporter Aequorin to Identify Novel Targets in Calcium Signaling

Published on: August 1, 2020

8.8K
In Vivo Functional Brain Imaging Approach Based on Bioluminescent Calcium Indicator GFP-aequorin
12:15

In Vivo Functional Brain Imaging Approach Based on Bioluminescent Calcium Indicator GFP-aequorin

Published on: January 8, 2016

13.0K
In Vitro Selection of Engineered Transcriptional Repressors for Targeted Epigenetic Silencing
10:44

In Vitro Selection of Engineered Transcriptional Repressors for Targeted Epigenetic Silencing

Published on: May 5, 2023

2.1K

Area of Science:

  • Biochemistry
  • Molecular Biology
  • Biotechnology

Background:

  • Luminescent proteins are explored for detection systems.
  • Bioluminescent proteins require no external excitation, ideal for analytical detection.
  • Aequorin is a photoprotein engineered as a sensitive molecular reporter.

Purpose of the Study:

  • To present characteristics of aequorin and its engineered variants.
  • To discuss the impact of aequorin variants on signal detection.
  • To highlight applications in biological sensing and imaging.

Main Methods:

  • Engineering and production of aequorin variants.
  • Characterization of structural features and activity of aequorin.
  • Evaluation of aequorin's performance in various detection systems.

Main Results:

  • Aequorin variants maintain sensitivity under diverse conditions.
  • Demonstrated benefits of aequorin as a label for sensing.
  • Successful applications in calcium detection, DNA/small molecule sensing, and in vivo imaging.

Conclusions:

  • Aequorin is a versatile bioluminescent protein for sensitive detection.
  • Engineered aequorin variants enhance signal detection in biological systems.
  • Aequorin facilitates insights into biological processes and advanced imaging.