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A novel sensitive cell-based Love Wave biosensor for marine toxin detection.
Xi Zhang1, Jiaru Fang1, Ling Zou1
1Biosensor National Special Lab, Key Lab for Biomedical Engineering of Ministry of Education, Department of Biomedical Engineering, Zhejiang University, 310027 Hangzhou, China.
Biosensors & Bioelectronics
|October 18, 2015
Summary
A new HepG2 cell biosensor detects Okadaic acid (OA), a diarrheic shellfish poisoning toxin. This acoustic wave sensor offers sensitive, real-time OA screening for shellfish safety.
Area of Science:
- Biomedical Engineering
- Biosensor Technology
- Analytical Chemistry
Background:
- Diarrheic Shellfish Poisoning (DSP) poses a significant public health risk.
- Okadaic acid (OA) is a primary toxin responsible for DSP.
- Sensitive and real-time detection methods for OA are crucial for food safety.
Purpose of the Study:
- To develop a novel HepG2 cell-based biosensor for the detection of Okadaic acid (OA).
- To utilize a Love Wave sensor for real-time monitoring of cellular responses to OA.
- To establish a convenient and sensitive platform for OA screening.
Main Methods:
- Fabrication of a Love Wave sensor using an ST-cut quartz substrate.
- Culturing HepG2 cells on the sensor surface to create a cell-based biosensor.
- Real-time monitoring of cell attachment and response to OA toxin using an 8-channel instrument.
Main Results:
- The biosensor successfully monitored HepG2 cell attachment in real time.
- Sensor response signals correlated with initial cell seeding densities.
- The cell-based biosensor demonstrated good performance for OA detection, with a linear range of 10-100 μg/L.
Conclusions:
- A novel HepG2 cell-based Love Wave biosensor enables real-time OA detection.
- The developed biosensor is a promising tool for convenient and sensitive OA screening.
- This acoustic wave platform offers a sensitive approach for monitoring toxin-induced cellular changes.
