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Related Experiment Video

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Vitrification of In Vitro Matured Oocytes Collected from Adult and Prepubertal Ovaries in Sheep
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Tretinoin-loaded lipid-core nanocapsules decrease reactive oxygen species levels and improve bovine embryonic

Caroline Gomes Lucas1, Mariana Härter Remião1, Eliza Rossi Komninou1

  • 1Programa de Pós-Graduação em Biotecnologia (PPGB), Laboratório de Embriologia Molecular e Transgenese, Grupo de Pesquisa em Oncologia Celular e Molecular, Biotecnologia/Centro de Desenvolvimento Tecnológico, Universidade Federal de Pelotas, Pelotas, 96010-610 RS, Brazil.

Reproductive Toxicology (Elmsford, N.Y.)
|October 18, 2015
PubMed
Summary

Lipid-core nanocapsules (TTN-LNC) improved in vitro oocyte maturation (IVM) by reducing toxic effects. This innovative approach enhanced blastocyst rates and decreased reactive oxygen species (ROS) and apoptosis markers.

Keywords:
Bovine oocytesIn vitro maturation (IVM)NanocapsulesNanoembryologySupplementationTretinoin

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Area of Science:

  • Reproductive Biology
  • Nanotechnology in Medicine
  • Developmental Biology

Background:

  • In vitro oocyte maturation (IVM) requires optimized culture media for improved outcomes.
  • Tretinoin (TTN), a retinoid crucial for embryonic development, faces challenges with solubility, stability, and toxicity.
  • Lipid-core nanocapsules (TTN-LNC) offer a novel method to enhance TTN delivery and mitigate its adverse effects.

Purpose of the Study:

  • To evaluate the efficacy of TTN-LNC as a supplement in IVM culture medium.
  • To assess the impact of TTN-LNC on reactive oxygen species (ROS) production, apoptosis markers, and gene expression in early embryonic development.
  • To determine if nanoencapsulation improves blastocyst rates and reduces tretinoin toxicity.

Main Methods:

  • Supplementation of IVM medium with varying concentrations of TTN-LNC and non-encapsulated TTN.
  • Analysis of reactive oxygen species (ROS) production, S36-phosphorylated-p66Shc levels, and caspase activity.
  • Quantitative assessment of apoptosis and pluripotency gene expression (BAX, SHC1) in blastocysts.

Main Results:

  • The lowest concentration of TTN-LNC (0.25μM) significantly increased blastocyst rates.
  • TTN-LNC supplementation led to reduced ROS production and lower S36-p66Shc levels compared to controls.
  • Expression of apoptosis-related genes (BAX) and SHC1 was decreased in both TTN and TTN-LNC treated groups, with nanoencapsulation enabling lower effective concentrations.

Conclusions:

  • Nanoencapsulation of tretinoin (TTN-LNC) is a promising strategy to enhance IVM protocols.
  • TTN-LNC reduces tretinoin-associated toxicity, including decreased ROS levels and apoptosis.
  • This approach improves early embryonic development outcomes, evidenced by higher blastocyst rates.