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Covalent Immobilization of Proteins for the Single Molecule Force Spectroscopy
Published on: August 20, 2018
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Efficient protein immobilization on polyethersolfone electrospun nanofibrous membrane via covalent binding for
Matin Mahmoudifard1, Sara Soudi2, Masoud Soleimani3
1Institute for Nanoscience and Nanotechnology, Sharif University of Technology, Tehran, Iran.
Summary
We developed a new antibody immobilization strategy using electrospun nanofibrous membranes for enhanced biosensor performance. This method improves detection signals in enzyme-linked immuno sorbent assay (ELISA) biosensors compared to traditional plates.
Area of Science:
- Biomaterials Science
- Biosensor Technology
- Surface Chemistry
Background:
- Antibody immobilization is crucial for biosensor development.
- Conventional methods often face limitations in efficiency and sensitivity.
- High surface area materials offer potential for improved antibody loading.
Purpose of the Study:
- To introduce a novel strategy for antibody immobilization using electrospun nanofibrous membranes.
- To evaluate the performance of these membranes for biosensor applications.
- To compare the efficiency of antibody immobilization with conventional methods.
Main Methods:
- Fabrication of polyethersulfone (PES) nanofibrous membranes.
- Surface activation using O2 plasma and ethyl-3-(3-dimethylaminopropyl)-carbodiimide/N-hydroxysuccinimide (EDC/NHS) coupling chemistry.
- Characterization using scanning electron microscopy (SEM), attenuated total reflectance Fourier transform infrared spectroscopy (ATR-FTIR), and atomic force microscopy (AFM).
- Quantification of antibody immobilization via enzyme-linked immuno sorbent assay (ELISA).
Main Results:
- Oxygen plasma treatment significantly enhanced antibody immobilization via EDC/NHS chemistry.
- Nanofibrous membranes showed improved antibody attachment compared to polyvinylidene fluoride (PVDF) membranes and microtiter plates.
- ELISA-based biosensors utilizing nanofibrous membranes exhibited enhanced detection signals.
Conclusions:
- The developed electrospun nanofibrous membrane strategy offers a high-performance platform for antibody immobilization.
- This technique holds potential for improving the sensitivity and efficiency of ELISA-based biosensors.
- The method is adaptable for various other biosensing applications.
