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Crystallization and preliminary X-ray studies of recombinant human granulocyte-macrophage colony stimulating factor
J M La Londe1, L S Hanna, R Rattoballi
1Chemistry Department, University of Pennsylvania, Philadelphia 19104.
Journal of Molecular Biology
|February 20, 1989
Abstract:
Human granulocyte-macrophage colony stimulating factor (hGM-CSF) is an important regulator of growth and differentiation for mononuclear and polymorphonuclear phagocytes. Here we report the crystallization and preliminary X-ray studies of Escherichia coli-expressed hGM-CSF. The crystals are orthorhombic, with the space group P212121, and have unit cell dimensions a = 46.62 A, b = 58.73 A and c = 126.42 A. Recombinant hGM-CSF crystals diffract X-rays to 2.4 A resolution and are thus suitable for X-ray structural studies.