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Cell-free Biochemical Fluorometric Enzymatic Assay for High-throughput Measurement of Lipid Peroxidation in High Density Lipoprotein
Published on: October 12, 2017
Lipoprotein subfraction oxidation in acute exercise and ageing
Paul Medlow1, Jane McEneny2, Marie H Murphy1
1a Sport and Exercise Science Research Institute , Ulster University , Jordanstown, Co. Antrim , Northern Ireland , UK ;
Acute exercise increases the oxidation of small, dense LDL particles, a process independent of age. However, older adults show a greater increase in LDL oxidation 24 hours post-exercise, indicating age is a factor in this response.
Area of Science:
- Exercise Physiology
- Cardiovascular Health
- Lipid Metabolism
Background:
- Exercise and aging independently elevate free radical production, potentially increasing LDL oxidation and atherogenesis.
- Understanding the impact of exercise and age on LDL subfractions' susceptibility to oxidation is crucial for cardiovascular disease risk assessment.
Purpose of the Study:
- To investigate the effects of acute exercise and aging on the oxidative susceptibility of LDL subfractions.
- To determine if age influences the changes in LDL lipid composition and oxidation following exercise.
Main Methods:
- Eleven aged (55±4 years) and twelve young (21±2 years) participants underwent a graded exercise test and a 1-hour moderate-intensity exercise bout.
- Blood samples were analyzed for lipid composition (cholesterol, triglycerides) and lipoprotein susceptibility to oxidation.
Main Results:
- Acute exercise increased small dense LDL (sdLDL) oxidation independently of age.
- sdLDL oxidation was further elevated 24 hours post-exercise in the aged group compared to the young group.
- Large buoyant LDL (lbLDL) in aged individuals showed elevated free cholesterol, total cholesterol, and triglycerides 24 hours post-exercise.
Conclusions:
- Acute exercise increases sdLDL oxidative susceptibility, irrespective of age or changes in LDL lipid composition.
- Age is a significant factor in the heightened susceptibility of sdLDL to oxidation 24 hours after exercise.
- While regular exercise is protective, acute exercise may transiently increase atherogenic risk, particularly in older adults.
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