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Assessment of Antibody-based Drugs Effects on Murine Bone Marrow and Peritoneal Macrophage Activation
Published on: December 26, 2017
An agonist antibody that blocks autoimmunity by inducing anti-inflammatory macrophages
Kyung Ho Han1, Rosana Gonzalez-Quintial1, Yingjie Peng1
1*Department of Cell and Molecular Biology and Department of Immunology and Microbial Science, The Scripps Research Institute, La Jolla, California, USA.
Abstract:
We have devised a method of using intracellular combinatorial libraries to select antibodies that control cell fates. Many agonist antibodies have been selected with this method, and the process appears to be limited only by the availability of a phenotypic selection system. We demonstrate the utility of this approach to discover agonist antibodies that engage an unanticipated target and regulate macrophage polarization by selective induction of anti-inflammatory M2 macrophages. This antibody was used therapeutically to block autoimmunity in a classic mouse model of spontaneous systemic lupus erythematosus.
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