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Published on: October 22, 2013
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Arcobacter butzleri isolates exhibit pathogenic potential in intestinal epithelial cell models
G Karadas1, R Bücker2, S Sharbati3
1Institute of Food Hygiene, Freie Universität Berlin, Berlin, Germany.
Journal of Applied Microbiology
|October 21, 2015
Summary
Arcobacter butzleri exhibits enteric pathogenic potential, demonstrating strain-specific interactions with human epithelial cells like adhesion and invasion. These mechanisms can disrupt the human intestinal barrier, potentially causing diarrhea.
Area of Science:
- Microbiology
- Cell Biology
- Gastroenterology
Background:
- Arcobacter butzleri is an emerging foodborne pathogen.
- Understanding its pathogenic mechanisms is crucial for public health.
Purpose of the Study:
- To investigate the pathogenic potential of Arcobacter butzleri isolates.
- To analyze the in vitro interactions with human (HT-29/B6) and porcine (IPEC-J2) epithelial cells.
Main Methods:
- In vitro assays were performed using human and porcine epithelial cell lines.
- Adhesion, invasion, cytotoxicity (WST assay), and epithelial barrier integrity (TER measurement) were assessed.
Main Results:
- Most Arcobacter butzleri isolates adhered to and invaded human cells, with lower rates in porcine cells.
- Cytotoxicity was observed in human cells, but not porcine cells.
- All isolates reduced epithelial barrier integrity in both cell types, more significantly in human cells.
Conclusions:
- Arcobacter butzleri possesses enteric pathogenic potential, with strain-specific mechanisms.
- Adhesion, invasion, and cytotoxicity contribute to human epithelial barrier dysfunction, potentially causing diarrhea.
- Porcine cells appear less susceptible to Arcobacter butzleri infection.
