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Updated: Mar 31, 2026

Highly Sensitive and Rapid Fluorescence Detection with a Portable FRET Analyzer
Published on: October 1, 2016
Water revealed as molecular mirror when measuring low concentrations of sugar with near infrared light
György Bázár1, Zoltan Kovacs2, Mariko Tanaka3
1Biomeasurement Technology Laboratory, Graduate School of Agricultural Science, Kobe University, Kobe, Japan; Institute of Food and Agricultural Product Qualification, Faculty of Agricultural and Environmental Sciences, Kaposvár University, Kaposvár, Hungary.
Abstract:
Near infrared spectroscopy is an overtone spectroscopy regarded as a quick and non-destructive method that provides analytical solutions for components that represent approximately 1% or more of the total mass of the investigated composite samples. Aquaphotomics offers the possibility for disentanglement of information remaining hidden in the spectra when conventional data evaluation methods are used, since this concept utilizes changes of the water structure induced by the measured solute as specific molecular vibrations at water bands. Here, near infrared technique and aquaphotomics are applied for non-destructive identification and quantification of mono- and di-saccharide solutes at 100-0.02 mM concentration that is accepted as unachievable with near infrared spectroscopy. The results presented in this study support the aquaphotomics' water molecular mirror concept that explores spectral changes related to water molecular rearrangements caused by minute changes of the solutes in the aqueous systems. The method provides quick and accurate alternative for classical analytical measurements of saccharides even at millimolar concentration levels.
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