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Genome-Wide Chromatin Immunoprecipitation in Candida albicans and Other Yeasts

Matthew B Lohse1, Pisiwat Kongsomboonvech2, Maria Madrigal2

  • 1Department of Microbiology and Immunology, University of California, San Francisco, 600 16th Street, San Francisco, CA, 94158, USA.

Methods in Molecular Biology (Clifton, N.J.)
|October 21, 2015
PubMed

Keywords:
Candida albicansChIP -chipChIP -seqChromatin immunoprecipitationYeast

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Chromatin Immunoprecipitation- ChIP02:36

Chromatin Immunoprecipitation- ChIP

Chromatin immunoprecipitation, or ChIP, is an antibody-based technique used to identify sites on DNA that bind to transcription factors of interest or histone proteins. It also helps determine the type of histone modifications such as acetylation, phosphorylation, or methylation.
Types of ChIP
ChIP can be divided into two types - X-ChIP and N-ChIP. X-ChIP involves in vivo cross-linking of histones and regulatory proteins to DNA, fragmenting the DNA by sonication, and isolating the protein-DNA...