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Related Experiment Video

Updated: Mar 31, 2026

Identification of protein complexes with quantitative proteomics in S. cerevisiae
11:12

Identification of protein complexes with quantitative proteomics in S. cerevisiae

Published on: March 4, 2009

13.7K

Label-Free Quantitative Proteomics in Yeast.

Thibaut Léger1, Camille Garcia1, Mathieu Videlier1

  • 1Mass Spectrometry Laboratory, Institut Jacques Monod, UMR7592, CNRS-Univ Paris Diderot, Sorbonne Paris Cité, Paris, France.

Methods in Molecular Biology (Clifton, N.J.)
|October 21, 2015
PubMed
Summary
This summary is machine-generated.

Label-free shotgun proteomics offers high-quality quantitative analysis of yeast proteomes using minimal protein. Optimizing protein extraction, instrument choice, quantification methods, and data analysis is crucial for robust yeast proteome dynamics insights.

Keywords:
Label-free quantificationMass spectrometryYeast proteomics

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Last Updated: Mar 31, 2026

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Area of Science:

  • Biochemistry
  • Proteomics
  • Yeast Biology

Background:

  • Quantitative proteomics enables deep understanding of cellular mechanisms.
  • Label-free shotgun mass spectrometry (MS) is a powerful technique for proteome analysis.
  • Analyzing the yeast proteome presents unique challenges and opportunities.

Purpose of the Study:

  • To present a robust workflow for label-free bottom-up shotgun MS-based proteomics in yeast.
  • To highlight key factors for optimizing quantitative proteomic analyses.
  • To provide insights into yeast proteome dynamics.

Main Methods:

  • Label-free bottom-up shotgun mass spectrometry.
  • Optimization of protein extract preparation.
  • Evaluation of instrument design and analytical capabilities.
  • Comparison of intensity-based versus spectral count quantification.
  • Application of appropriate quantification algorithms.

Main Results:

  • Demonstration of high-quality quantitative analysis of the yeast proteome with microgram protein amounts.
  • Identification of critical parameters influencing quantitative accuracy and reproducibility.
  • Establishment of a comprehensive workflow for data acquisition, processing, and analysis.

Conclusions:

  • Label-free shotgun MS is a powerful and accessible tool for yeast proteome quantification.
  • Careful consideration of methodological factors is essential for maximizing the technique's potential.
  • The developed workflow offers unprecedented insights into yeast proteome dynamics.