Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Alternative RNA Splicing02:18

Alternative RNA Splicing

26.4K
Alternative RNA splicing is the regulated splicing of exons and introns to produce different mature mRNAs from a single pre-mRNA. Unlike in constitutive splicing where a single gene produces a single type of mRNA, alternative splicing allows an organism to produce multiple proteins from a single gene and plays an important role in protein diversity.
There are five types of alternative RNA splicing that vary in the ways the pre-mRNA segments are removed or retained in the mature mRNA. The first...
26.4K
Alternative RNA Splicing02:18

Alternative RNA Splicing

5.5K
5.5K
Comparing Copy Number Variations and SNPs02:26

Comparing Copy Number Variations and SNPs

19.3K
Sequencing of the human genome has opened up several best-kept secrets of the genome. Scientists have identified thousands of genome variations that exist within a population. These variations can be a single nucleotide or a larger chromosomal variation.
Copy number variations or CNVs are the structural variations that cover more than 1kb of DNA sequence. The single nucleotide polymorphism (SNP), on the other hand, is a single nucleotide change or a point mutation that is found in more than 1%...
19.3K
RNA Splicing01:32

RNA Splicing

61.4K
Splicing is the process by which eukaryotic RNA is edited before its translation into protein. The RNA strand transcribed from eukaryotic DNA is called the primary transcript. The primary transcripts that become mRNAs are called precursor messenger RNAs (pre-mRNAs). Eukaryotic pre-mRNA contains alternating sequences of exons and introns. Exons are nucleotide sequences that code for proteins, whereas introns are the non-coding regions. In RNA splicing, introns are removed and exons are bonded...
61.4K
RNA Splicing01:32

RNA Splicing

20.3K
20.3K
Pre-mRNA Processing: RNA Splicing01:36

Pre-mRNA Processing: RNA Splicing

7.4K
7.4K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Antibiotic non-susceptibility associated serotypes of invasive pneumococcal disease - a nationwide population study from Switzerland, 2012-2022.

Pneumonia (Nathan Qld.)·2026
Same author

Referral pathway for adults with chronic cough in Switzerland from a Delphi-based consensus study.

Postgraduate medicine·2026
Same author

Temporal Trends and Burden of Hospitalizations for Presumed Cardiac Sarcoidosis.

Respiration; international review of thoracic diseases·2026
Same author

Effect of electronic nicotine delivery systems for smoking cessation on sleep quality: secondary analysis of a randomized controlled trial.

Sleep·2026
Same author

Smartphone-Enabled Cough Detection in Severely Exacerbated COPD: An Exploratory Pilot Study.

COPD·2026
Same author

Continuous SpO<sub>2</sub> Monitoring Using Reflectance Pulse Oximetry at the Wrist and Upper Arm During Overnight Sleep Apnea Recordings.

Annual International Conference of the IEEE Engineering in Medicine and Biology Society. IEEE Engineering in Medicine and Biology Society. Annual International Conference·2025

Related Experiment Video

Updated: Mar 31, 2026

Using RNA-sequencing to Detect Novel Splice Variants Related to Drug Resistance in In Vitro Cancer Models
09:58

Using RNA-sequencing to Detect Novel Splice Variants Related to Drug Resistance in In Vitro Cancer Models

Published on: December 9, 2016

14.5K

High-throughput alternative splicing detection using dually constrained correspondence analysis (DCCA).

Florent Baty1, Dirk Klingbiel2, Francesco Zappa3

  • 1Department of Pulmonary Medicine, Cantonal Hospital St. Gallen, Switzerland.

Journal of Biomedical Informatics
|October 21, 2015
PubMed
Summary
This summary is machine-generated.

This study introduces Dually Constrained Correspondence Analysis (DCCA) to detect alternative splicing changes in cancer. The method identified key genes involved in tumor growth and suppression in non-small cell lung cancer patients.

Keywords:
Alternative splicingCorrespondence analysisLung cancerMultivariate statisticsTargeted therapies

More Related Videos

Identification of Alternative Splicing and Polyadenylation in RNA-seq Data
08:35

Identification of Alternative Splicing and Polyadenylation in RNA-seq Data

Published on: June 24, 2021

6.6K
Quantitative Analysis of Alternative Pre-mRNA Splicing in Mouse Brain Sections Using RNA In Situ Hybridization Assay
11:22

Quantitative Analysis of Alternative Pre-mRNA Splicing in Mouse Brain Sections Using RNA In Situ Hybridization Assay

Published on: August 26, 2018

9.4K

Related Experiment Videos

Last Updated: Mar 31, 2026

Using RNA-sequencing to Detect Novel Splice Variants Related to Drug Resistance in In Vitro Cancer Models
09:58

Using RNA-sequencing to Detect Novel Splice Variants Related to Drug Resistance in In Vitro Cancer Models

Published on: December 9, 2016

14.5K
Identification of Alternative Splicing and Polyadenylation in RNA-seq Data
08:35

Identification of Alternative Splicing and Polyadenylation in RNA-seq Data

Published on: June 24, 2021

6.6K
Quantitative Analysis of Alternative Pre-mRNA Splicing in Mouse Brain Sections Using RNA In Situ Hybridization Assay
11:22

Quantitative Analysis of Alternative Pre-mRNA Splicing in Mouse Brain Sections Using RNA In Situ Hybridization Assay

Published on: August 26, 2018

9.4K

Area of Science:

  • Genomics
  • Bioinformatics
  • Cancer Biology

Background:

  • Alternative splicing plays a crucial role in tumorigenesis.
  • Exon array technology allows for genome-wide detection of alternative splicing.
  • Standardized methods for analyzing high-throughput alternative splicing data are still developing.

Purpose of the Study:

  • To propose and validate a novel statistical approach, Dually Constrained Correspondence Analysis (DCCA), for detecting alternative splicing changes in exon array data.
  • To investigate genome-wide alternative splicing alterations in non-small cell lung cancer (NSCLC) patients treated with bevacizumab/erlotinib.

Main Methods:

  • Development and application of Dually Constrained Correspondence Analysis (DCCA).
  • Analysis of high-throughput exon array data from NSCLC patients.
  • Identification and characterization of genes with altered alternative splicing.

Main Results:

  • DCCA successfully identified significant alternative splicing events in NSCLC.
  • Detected genes are implicated in various cancer-related processes including carcinogenesis, cell adhesion, tumor aggressiveness, apoptosis, proliferation, differentiation, invasion, growth, necrosis, and suppression.
  • Identified genes include SFTPB, STAB2, PCDH15, HABP2, ARNTL2, PDE4D, FLT3, IL1R2, ETV1, OLFM4, FGF14, AFF3, TUSC3, CSMD1, RHOBTB2, and SERPINB5.
  • A majority of the identified genes showed known alternative splicing events.

Conclusions:

  • DCCA is an effective statistical method for identifying biologically relevant alternative splicing events in high-throughput exon array data.
  • The study highlights the significant role of alternative splicing alterations in non-small cell lung cancer.
  • The identified genes provide potential targets for understanding and treating NSCLC.