Dataset of natural antisense transcripts in P. vivax clinical isolates derived using custom designed strand-specific...

Area of Science:

• Genomics
• Molecular Biology
• Parasitology

Background:

• Natural antisense transcripts (NATs) regulate gene expression across organisms.
• NATs are known in Plasmodium falciparum, but their presence in Plasmodium vivax was unconfirmed.
• Plasmodium vivax causes severe malaria, similar to P. falciparum.

Purpose of the Study:

• To investigate the in vivo prevalence of antisense transcripts in clinical isolates of Plasmodium vivax.
• To establish a resource for exploring NATs in P. vivax.

Main Methods:

• Whole genome expression profiling was performed.
• A custom-designed, strand-specific microarray with probes for sense and antisense strands was utilized.
• Microarray data analysis was conducted and deposited in Gene Expression Omnibus (GEO) under GSE45165.

Main Results:

• The study successfully identified the presence of antisense transcripts in Plasmodium vivax clinical isolates.
• Expression profiling revealed NATs in P. vivax, contributing to the understanding of its gene regulation.
• The data provides a foundation for further research into NATs in P. vivax.

Conclusions:

• Antisense transcripts are present in Plasmodium vivax.
• This finding expands the known regulatory mechanisms in malaria parasites.
• The study provides a valuable resource for future research on P. vivax gene expression and pathogenesis.