Related Experiment Video
Updated: Mar 31, 2026
In Vivo Biosensor Tracks Non-apoptotic Caspase Activity in Drosophila
Published on: November 27, 2016
Transcriptional profiling of apoptosis-deficient Drosophila mutants
Fumiaki Obata1, Katsura Tomioka1, Masayuki Miura2
1Department of Genetics, Graduate School of Pharmaceutical Sciences, The University of Tokyo, Bunkyo-ku, Tokyo 113-0033, Japan.
Abstract:
Apoptosis is a fundamental way to remove damaged or unwanted cells during both developmental and post-developmental stages. Apoptosis deficiency leads to various diseases including cancer. To know the physiological changes in apoptosis-deficient mutants, we conducted non-biased transcriptomic analysis of Drosophila dark(cd4) mutants. As recently reported, combined with metabolome and genetic analysis, we identified systemic immune response, energy wasting, as well as alteration in S-adenosyl-methionine metabolism in response to necrotic cells [1]. Here, we describe in detail how we obtained validated microarray dataset deposited in Gene Expression Omnibus (GSE47853). Our data provide a resource for searching transcriptional alterations in Drosophila apoptosis-deficient mutants.
Insights
Apoptosis deficiency in Drosophila mutants causes systemic immune response and energy wasting. This study details the microarray dataset (GSE47853) for investigating these physiological changes.
Area of Science:
- Cell Biology
- Genetics
- Developmental Biology
Background:
- Apoptosis is crucial for removing damaged cells and preventing diseases like cancer.
- Defects in apoptosis are linked to various pathological conditions, including tumorigenesis.
- Understanding apoptosis-deficient mutants provides insights into cellular homeostasis and disease mechanisms.
Purpose of the Study:
- To investigate the physiological consequences of apoptosis deficiency in Drosophila.
- To identify transcriptional alterations in apoptosis-deficient mutants using transcriptomic analysis.
- To provide a validated microarray dataset as a resource for future research.
Main Methods:
- Non-biased transcriptomic analysis (microarray) of Drosophila dark(cd4) mutants.
- Data validation and deposition in the Gene Expression Omnibus (GEO) under accession number GSE47853.
- Integration with existing metabolome and genetic analyses.
Main Results:
- Identification of a systemic immune response in apoptosis-deficient mutants.
- Observation of significant energy wasting.
- Detection of alterations in S-adenosyl-methionine metabolism.
- Characterization of transcriptional changes associated with necrotic cell response.
Conclusions:
- The study provides a comprehensive transcriptomic dataset (GSE47853) for Drosophila apoptosis-deficient mutants.
- The findings highlight the complex physiological responses, including immune activation and metabolic dysregulation, to apoptosis deficiency.
- The data serves as a valuable resource for researchers studying cell death, disease, and developmental processes.
