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Updated: Mar 31, 2026

Quantitative Analysis of Alternative Pre-mRNA Splicing in Mouse Brain Sections Using RNA In Situ Hybridization Assay
Published on: August 26, 2018
Genome-wide profiling of alternative splicing in Alzheimer's disease
Mitchell K P Lai1, Margaret M Esiri2, Michelle G K Tan3
1Department of Pharmacology, Yong Loo Lin School of Medicine, National University of Singapore, Singapore.
Abstract:
Alternative splicing is a highly regulated process which generates transcriptome and proteome diversity through the skipping or inclusion of exons within gene loci. Identification of aberrant alternative splicing associated with human diseases has become feasible with the development of new genomic technologies and powerful bioinformatics. We have previously reported genome-wide gene alterations in the neocortex of a well-characterized cohort of Alzheimer's disease (AD) patients and matched elderly controls using a commercial exon microarray platform [1]. Here, we provide detailed description of analyses aimed at identifying differential alternative splicing events associated with AD.
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