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Transcriptomic analyses of Onecut1 and Onecut2 deficient retinas
Jillian J Goetz1, Jeffrey M Trimarchi1
1Department of Genetics, Development and Cell Biology, 2114 Molecular Biology, Iowa State University, Ames, IA 50011, USA.
Genomics Data
|October 21, 2015
Summary
Onecut1 and Onecut2 transcription factors are crucial for mouse retina development. This study details gene expression changes in retinas lacking these factors, providing valuable data for retinogenesis research.
Area of Science:
- Developmental Biology
- Genomics
- Neuroscience
Background:
- The Onecut family of transcription factors, including Onecut1 and Onecut2, are implicated in developmental processes.
- Understanding their specific roles in the complex development of the mammalian retina is essential.
Purpose of the Study:
- To comprehensively analyze the transcriptomes of developing and mature mouse retinas.
- To identify genes with differential expression in the absence of Onecut1 or Onecut2.
- To provide a detailed dataset for further research into retinogenesis.
Main Methods:
- RNA sequencing (Affymetrix microarrays) was performed on wildtype and Onecut1/2 deficient mouse retinas.
- Differential gene expression analysis was conducted to compare transcriptomes.
- Raw data is publicly accessible via NCBI Gene Expression Omnibus (GEO).
Main Results:
- Comprehensive transcriptome data from developing and mature mouse retinas were generated.
- Tables detailing differentially expressed genes between wildtype and mutant retinas are provided.
- This dataset highlights genes regulated by Onecut1 and Onecut2 during retinal development.
Conclusions:
- The data presented offers a valuable resource for studying the molecular mechanisms of retinogenesis.
- The findings underscore the critical roles of Onecut1 and Onecut2 in regulating gene expression during retinal development.
- Further investigation into the identified differentially expressed genes can elucidate specific developmental pathways.
