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Related Experiment Video

Updated: Mar 31, 2026

Isolation of Human Lymphatic Endothelial Cells by Multi-parameter Fluorescence-activated Cell Sorting
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A microarray analysis of two distinct lymphatic endothelial cell populations.

Bernhard Schweighofer1, Sabrina Rohringer2, Johannes Pröll3

  • 1Skin and Endothelium Research Division, Department of Dermatology, Medical University of Vienna, Vienna, Austria.

Genomics Data
|October 21, 2015
PubMed
Summary

Lymphatic endothelial cells (LECs) were found to exist in two distinct populations with varying podoplanin levels. Shockwave treatment enriched the podoplanin-high LECs, enhancing their proliferation and migration capabilities.

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Area of Science:

  • Cell Biology
  • Lymphatic System Research
  • Biotechnology

Background:

  • Lymphatic endothelial cells (LECs) are crucial for lymphatic system function.
  • Two distinct LEC populations with differential podoplanin expression have been identified.
  • Podoplanin is a key surface marker for LECs.

Purpose of the Study:

  • To provide detailed information on a dataset (GSE62510) characterizing two LEC populations.
  • To describe the analysis methods used to identify differentially expressed genes between these LEC populations.
  • To investigate the effects of in vitro shockwave treatment (IVSWT) on LEC populations.

Main Methods:

  • Isolation and characterization of two distinct LEC populations based on podoplanin expression.
  • In vitro shockwave treatment (IVSWT) of LECs.
  • Affymetrix microarray analysis to determine gene expression profiles.
  • Bioinformatic analysis to identify differentially expressed genes.

Main Results:

  • LECs form two morphologically distinct populations with differing podoplanin levels.
  • IVSWT enriched the podoplanin-high LEC population.
  • Enriched podoplanin-high LECs showed increased proliferation and migration (2D and 3D).
  • Gene expression profiling revealed distinct molecular signatures for each LEC population.

Conclusions:

  • LECs exist as heterogeneous populations with distinct functional properties.
  • IVSWT can modulate LEC populations, favoring the enrichment of a more proliferative and migratory phenotype.
  • The identified gene expression differences provide insights into the molecular mechanisms underlying LEC heterogeneity and response to stimuli.