Related Experiment Video
Updated: Mar 31, 2026

DNA-affinity-purified Chip DAP-chip Method to Determine Gene Targets for Bacterial Two component Regulatory Systems
Published on: July 21, 2014
Genome-wide DNA binding pattern of two-component system response regulator RhpR in Pseudomonas syringae
Tianhong Zhou1, Kai Chen2, Hai-Xin Zhang3
1TEDA Institute of Biological Sciences and Biotechnology, Nankai University, 23 Hongda Street, Tianjin 300457, PR China ; Key Laboratory of Molecular Microbiology and Technology, Ministry of Education, Tianjin 300071, PR China.
Abstract:
Although Pseudomonas syringae uses the two-component system RhpRS to modulate the expression of type III secretion system (T3SS) genes and pathogenicity, the molecular mechanisms and the regulon of RhpRS have yet to be fully demonstrated. We have performed a genome-wide analysis of RhpR binding to DNA prepared from P. syringae pv. phaseolicola in order to identify candidate direct targets of RhpR-mediated transcriptional regulation, as described in our recent article [1]. The data are available from NCBI Gene Expression Omnibus (GEO) with the accession number GSE58533. Here we describe the detailed methods and data analyses of our RhpR ChIP-seq dataset.
Related Concept Videos
Global Regulatory Systems
Other Stress Responses in Bacteria
Stringent Response in E. coli
Regulation of Bacterial Virulence
Cooperative Binding of Transcription Regulators
Prokaryotic Transcriptional Activators and Repressors
Transcription of prokaryotic...

