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Updated: Mar 31, 2026
Author Spotlight: Analyzing Bone Marrow Microenvironment in Murine Hematological Malignancies
Published on: November 10, 2023
Transcriptome analysis of bone marrow mesenchymal stromal cells from patients with primary myelofibrosis
Christophe Martinaud1, Christophe Desterke1, Johanna Konopacki1
1INSERM Unit 1197 and UMS33, Paul Brousse Hospital, Andre Lwoff Institute, France.
Abstract:
Primary myelofibrosis (PMF) is a clonal myeloproliferative neoplasm whose severity and treatment complexity are attributed to the presence of bone marrow (BM) fibrosis and alterations of stroma impairing the production of normal blood cells. Despite the recently discovered mutations including the JAK2V617F mutation in about half of patients, the primitive event responsible for the clonal proliferation is still unknown. In the highly inflammatory context of PMF, the presence of fibrosis associated with a neoangiogenesis and an osteosclerosis concomitant to the myeloproliferation and to the increase number of circulating hematopoietic progenitors suggests that the crosstalk between hematopoietic and stromal cells is deregulated in the PMF BM microenvironmental niches. Within these niches, mesenchymal stromal cells (BM-MSC) play a hematopoietic supportive role in the production of growth factors and extracellular matrix which regulate the proliferation, differentiation, adhesion and migration of hematopoietic stem/progenitor cells. A transcriptome analysis of BM-MSC in PMF patients will help to characterize their molecular alterations and to understand their involvement in the hematopoietic stem/progenitor cell deregulation that features PMF.
Insights
Primary myelofibrosis involves bone marrow fibrosis that disrupts blood cell production. This study investigates how bone marrow mesenchymal stromal cells are altered in PMF to understand their role in disease progression.
Area of Science:
- Hematology
- Oncology
- Cell Biology
Background:
- Primary myelofibrosis (PMF) is a myeloproliferative neoplasm characterized by bone marrow fibrosis, impacting normal blood cell production.
- While mutations like JAK2V617F are known, the initial cause of clonal proliferation in PMF remains unidentified.
- The PMF microenvironment exhibits inflammation, fibrosis, neoangiogenesis, and osteosclerosis, suggesting deregulated crosstalk between hematopoietic and stromal cells.
Purpose of the Study:
- To characterize molecular alterations in bone marrow mesenchymal stromal cells (BM-MSC) from PMF patients.
- To elucidate the role of these altered BM-MSCs in the deregulation of hematopoietic stem and progenitor cells observed in PMF.
Main Methods:
- Transcriptome analysis of bone marrow mesenchymal stromal cells (BM-MSC) isolated from primary myelofibrosis patients.
Main Results:
- Transcriptome analysis is expected to reveal specific molecular alterations within PMF BM-MSCs.
- These findings will provide insights into how BM-MSC dysfunction contributes to hematopoietic stem/progenitor cell deregulation in PMF.
Conclusions:
- Understanding BM-MSC alterations is crucial for deciphering the complex pathophysiology of primary myelofibrosis.
- This research may identify novel therapeutic targets within the bone marrow microenvironment for PMF treatment.
