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Differentiation and Characterization of Neural Progenitors and Neurons from Mouse Embryonic Stem Cells
Published on: May 15, 2020
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Genome-wide copy number profiling of mouse neural stem cells during differentiation
U Fischer1, N Ludwig1, A Keller2
1Department of Human Genetics, Saarland University, Building 60, 66421 Homburg/Saar, Germany.
Genomics Data
|October 21, 2015
Summary
Gene amplifications occur in mouse neural stem cells during differentiation. This study details copy number changes, including gene amplifications and deletions, observed during neural stem cell differentiation.
Area of Science:
- Genetics
- Developmental Biology
- Stem Cell Biology
Background:
- Gene amplifications have been observed in neural stem and progenitor cells during differentiation.
- Understanding these changes is crucial for developmental processes.
Purpose of the Study:
- To investigate copy number variations, including gene amplifications and deletions, during mouse neural stem cell differentiation.
- To detail cell culture methods and validation experiments for array comparative genomic hybridization (array-CGH) analysis.
Main Methods:
- Array comparative genomic hybridization (array-CGH) was employed to detect copy number changes.
- TaqMan quantitative real-time PCR (qPCR) was used to validate array-CGH findings.
- Neural stem cells were differentiated using transforming growth factor-beta (TGF-ß) and fetal calf serum (FCS).
Main Results:
- Copy number changes, including gene amplifications and deletions, were identified during neural stem cell differentiation.
- Array data were deposited in the Gene Expression Omnibus (GEO) under accession number GSE35523.
- Detailed analysis of amplified and deleted chromosome regions was performed.
Conclusions:
- The study provides a timeline of copy number changes during neural stem cell differentiation.
- The findings contribute to understanding genetic alterations in neural stem cells during development.
