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Culturing and Manipulation of O9-1 Neural Crest Cells
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Transcriptomic profiling comparison of YAP over-expression and conditional knockout mouse tooth germs.

Ming Liu1, Xiu-Ping Wang1

  • 1Department of Developmental Biology, Harvard School of Dental Medicine, Boston, MA 02115, USA.

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|October 21, 2015
PubMed
Summary

Yes-associated protein (YAP) regulates downstream target genes, including Hox, Wnt, and Laminin families, in mouse tooth development. This study details the transcriptomic profiling of YAP manipulation in mouse tooth germs.

Keywords:
HoxLamininRNA-seqWntYAP

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Area of Science:

  • Developmental Biology
  • Molecular Biology
  • Genomics

Background:

  • The Hippo-YAP signaling pathway is crucial for organ size control and tissue homeostasis.
  • Understanding YAP's downstream targets is essential for elucidating its role in development.

Purpose of the Study:

  • To identify downstream target genes regulated by YAP during mouse tooth germ development.
  • To characterize the transcriptomic changes associated with YAP manipulation.

Main Methods:

  • RNA sequencing (RNA-Seq) was employed to compare gene expression profiles.
  • Transcriptomic profiling was performed on Yap conditional knockout (Yap CKO) and YAP over-expression mouse tooth germs.

Main Results:

  • Concurrent changes in YAP transcripts were observed with genes from the Hox, Wnt, and Laminin families.
  • These findings suggest YAP regulates the expression of these gene families.

Conclusions:

  • YAP plays a significant role in regulating key developmental gene families during tooth morphogenesis.
  • The study provides detailed transcriptomic data (NCBI GEO accession number GSE65524) for further research.