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Transcriptomic profiling comparison of YAP over-expression and conditional knockout mouse tooth germs
1Department of Developmental Biology, Harvard School of Dental Medicine, Boston, MA 02115, USA.
Abstract:
To identify the downstream target genes of YAP, we used RNA-Seq technology to compare the transcriptomic profilings of Yap conditional knockout (Yap CKO) and YAP over-expression mouse tooth germs. Our results showed that some Hox, Wnt and Laminin family genes had concurrent changes with YAP transcripts, indicating that the expression of these genes may be regulated by YAP. Here, we provide the detailed experimental procedure for the transcriptomic profiling results (NCBI GEO accession number GSE65524). The associated study on the regulation of Hoxa1 and Hoxc13 genes by YAP was published in Molecular Cellular Biology in 2015 [Liu et al., 2015].
Insights
Yes-associated protein (YAP) regulates downstream target genes, including Hox, Wnt, and Laminin families, in mouse tooth development. This study details the transcriptomic profiling of YAP manipulation in mouse tooth germs.
Area of Science:
- Developmental Biology
- Molecular Biology
- Genomics
Background:
- The Hippo-YAP signaling pathway is crucial for organ size control and tissue homeostasis.
- Understanding YAP's downstream targets is essential for elucidating its role in development.
Purpose of the Study:
- To identify downstream target genes regulated by YAP during mouse tooth germ development.
- To characterize the transcriptomic changes associated with YAP manipulation.
Main Methods:
- RNA sequencing (RNA-Seq) was employed to compare gene expression profiles.
- Transcriptomic profiling was performed on Yap conditional knockout (Yap CKO) and YAP over-expression mouse tooth germs.
Main Results:
- Concurrent changes in YAP transcripts were observed with genes from the Hox, Wnt, and Laminin families.
- These findings suggest YAP regulates the expression of these gene families.
Conclusions:
- YAP plays a significant role in regulating key developmental gene families during tooth morphogenesis.
- The study provides detailed transcriptomic data (NCBI GEO accession number GSE65524) for further research.
