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Updated: Mar 31, 2026
Evaluation of Lung Metastasis in Mouse Mammary Tumor Models by Quantitative Real-time PCR
Published on: January 29, 2016
Deep sequencing of mRNA in CD24(-) and CD24(+) mammary carcinoma Mvt1 cell line
Ran Rostoker1, Anitha D Jayaprakash2, Ravi Sachidanandam3
1Diabetes and Metabolism Clinical Research Center of Excellence, Clinical Research Institute at Rambam (CRIR), Haifa 31096, Israel ; Faculty of Medicine, Technion, Rambam Medical Center, Haifa 31096, Israel.
Abstract:
CD24 is an anchored cell surface marker that is highly expressed in cancer cells (Lee et al., 2009) and its expression is associated with poorer outcome of cancer patients (Kristiansen et al., 2003). Phenotype comparison between two subpopulations derived from the Mvt1 cell line, CD24(-) cells (with no CD24 cell surface expression) and the CD24(+) cells, identified high tumorigenic capacity for the CD24(+) cells. In order to reveal the transcripts that support the CD24(+) aggressive and invasive phenotype we compared the gene profiles of these two subpopulations. mRNA profiles of CD24(-) and CD24(+) cells were generated by deep sequencing, in triplicate, using an Illumina HiSeq 2500. Here we provide a detailed description of the mRNA-seq analysis from our recent study (Rostoker et al., 2015). The mRNA-seq data have been deposited in the NCBI GEO database (accession number GSE68746).
