Modification of collagen-chitosan matrix by the natural crosslinker alginate dialdehyde

In the present study, collagen (Coll) was mixed with the natural crosslinker chitosan (CTS), and then, alginate dialdehyde (ADA) was added to crosslink the mixtures. The properties of these Coll matrix sponges were investigated afterwards. Fourier transform infrared spectroscopy (FTIR) analysis and in vitro fiber formation analysis showed the intact retention of the classical triple-helical structure after crosslinking. Scanning electron microscopy (SEM) showed that microfibril structural interactions between Coll structures became more compact. Significant improvement in the thermostability of the crosslinked mixtures was observed with the pure mixtures of Coll and CTS. Antibacterial activity measurements indicated no affect of ADA on Coll/CTS sponges. In conclusion, the modification of the Coll/CTS mixtures with ADA preserves the classical triple-helical structure, enhances stabilization, maintains good biocompatibility and may pave the way for new medical applications.