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Related Experiment Video

Updated: Feb 8, 2026

Lumican Extraction from Amniotic Membrane and Determination of its Storage Temperature

Lumican Extraction from Amniotic Membrane and Determination of its Storage Temperature

Published on: October 14, 2022

Membrane Transition Temperature Determines Cisplatin Response.

Krishnan Raghunathan¹, Aarif Ahsan², Dipankar Ray²

¹Department of Biophysics, University of Michigan, Ann Arbor, Michigan, United States of America.

|October 21, 2015

Summary

This summary is machine-generated.

Cisplatin resistance in cancer may stem from altered plasma membrane properties. Modulating membrane heterogeneity with agents like isopropanol or menthol can enhance or reduce cisplatin sensitivity, offering new therapeutic strategies.

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Area of Science:

Biochemistry
Cell Biology
Cancer Research

Background:

Cisplatin is a key chemotherapy for cancers like head and neck cancer.
Mechanisms of cisplatin resistance are not fully understood.
Plasma membrane biophysical properties may influence drug response.

Purpose of the Study:

To investigate the role of plasma membrane heterogeneity in cisplatin resistance.
To determine if changes in membrane properties are a cause or consequence of resistance.
To explore therapeutic strategies targeting membrane properties.

Main Methods:

Utilized giant plasma membrane vesicles (GPMVs) isolated from cells.
Assessed miscibility transition temperature of GPMVs as a measure of membrane heterogeneity.
Tested the effect of cisplatin, isopropanol, and menthol on GPMV transition temperatures and cell sensitivity.

Main Results:

Cisplatin lowered GPMV transition temperature in sensitive cells but not resistant cells.
Isopropanol increased cisplatin sensitivity by lowering GPMV transition temperature.
Menthol decreased cisplatin sensitivity by raising GPMV transition temperature.

Conclusions:

Plasma membrane heterogeneity plays a significant role in determining cisplatin response.
Modulating membrane heterogeneity can overcome cisplatin resistance.
Targeting membrane biophysical properties presents a potential therapeutic avenue.