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Decrease in an Inwardly Rectifying Potassium Conductance in Mouse Mammary Secretory Cells after Forced Weaning.

Akihiro Kamikawa1, Shota Sugimoto1, Osamu Ichii2

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Summary

Weaning reduces inwardly rectifying potassium channel (Kir) 2.1 current and expression in mouse mammary secretory cells. This decrease in Kir conductance may stop ion secretion into milk after lactation.

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Area of Science:

  • Mammalian physiology
  • Molecular biology
  • Ion channel function

Background:

  • Mammary glands are active during lactation and involute post-weaning.
  • Inwardly rectifying potassium channels (Kir) are present in lactating mammary secretory (MS) cells.
  • Kir channels may influence milk ion content.

Purpose of the Study:

  • To investigate the regulation of Kir channels in mouse mammary glands after weaning.
  • To determine the effect of forced weaning on Ba2+-sensitive Kir current and Kir2.1 expression.

Main Methods:

  • Histological analysis of mammary acini post-weaning.
  • Whole-cell patch-clamp electrophysiology to measure Kir current.
  • Real-time PCR and Western blot to assess Kir2.1 mRNA and protein levels.
  • Experimental induction of milk stasis via teat sealing.

Main Results:

  • Post-weaning MS cells exhibited significantly reduced Ba2+-sensitive Kir current (0.93 nS) compared to lactating cells (4.25 nS).
  • Kir2.1 mRNA and protein expression decreased by 90% and 47%, respectively, after weaning.
  • Milk stasis induced by teat sealing also reduced Kir conductance (0.36 nS) and Kir2.1 mRNA expression.

Conclusions:

  • Milk stasis immediately following weaning decreases Kir conductance in MS cells.
  • Reduced Kir2.1 expression contributes to the diminished Kir conductance post-weaning.
  • These changes likely play a role in the cessation of ion secretion into milk.