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High Throughput Single-cell and Multiple-cell Micro-encapsulation

High Throughput Single-cell and Multiple-cell Micro-encapsulation

Published on: June 15, 2012

One-to-one encapsulation based on alternating droplet generation.

Hirotada Hirama¹, Toru Torii¹

¹Department of Human and Engineered Environmental Studies, Graduate School of Frontier Sciences, The University of Tokyo, Kashiwa-shi, Chiba 277-8563, Japan.

Scientific Reports

|October 22, 2015

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Researchers developed a microfluidic method for precise one-to-one encapsulation of cell models. This technique controls particle numbers in droplets, enabling applications in single-cell assays and cell fusion.

Area of Science:

Biotechnology
Microfluidics
Materials Science

Background:

Cell encapsulation is crucial for various biological applications.
Achieving precise control over the number of encapsulated particles per droplet remains a challenge.

Purpose of the Study:

To develop a microfluidic method for controlled one-to-one encapsulation of particles as cell models.
To demonstrate the utility of this method for potential applications in single-cell analysis and cell manipulation.

Main Methods:

Utilized an alternating droplet generation encapsulation technique.
Employed a microchannel with locally treated wettability for uniform droplet formation.
Incorporated spontaneous droplet coalescence using specific surfactants for particle enclosure.

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Last Updated: Mar 31, 2026

High Throughput Single-cell and Multiple-cell Micro-encapsulation

High Throughput Single-cell and Multiple-cell Micro-encapsulation

Published on: June 15, 2012

Title Cell Encapsulation by Droplets

Title Cell Encapsulation by Droplets

Published on: October 1, 2007

A Pipette-Tip Based Method for Seeding Cells to Droplet Microfluidic Platforms

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Published on: February 11, 2019

Main Results:

Successfully encapsulated fluorescent particles as cell models in uniform water-in-oil-in-water (W/O/W) emulsion droplets.
Achieved up to 20% of particle-containing droplets with single-particle encapsulation.
Demonstrated successful enclosure of particles within inner aqueous droplets via droplet coalescence.

Conclusions:

The alternating droplet generation method enables precise one-to-one encapsulation.
This technique offers a versatile platform for applications like high-throughput single-cell assays, gene transfection, and cell fusion.