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Related Experiment Video

Updated: Mar 31, 2026

High Throughput Single-cell and Multiple-cell Micro-encapsulation
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One-to-one encapsulation based on alternating droplet generation.

Hirotada Hirama1, Toru Torii1

  • 1Department of Human and Engineered Environmental Studies, Graduate School of Frontier Sciences, The University of Tokyo, Kashiwa-shi, Chiba 277-8563, Japan.

Scientific Reports
|October 22, 2015
PubMed
Summary

Researchers developed a microfluidic method for precise one-to-one encapsulation of cell models. This technique controls particle numbers in droplets, enabling applications in single-cell assays and cell fusion.

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Area of Science:

  • Biotechnology
  • Microfluidics
  • Materials Science

Background:

  • Cell encapsulation is crucial for various biological applications.
  • Achieving precise control over the number of encapsulated particles per droplet remains a challenge.

Purpose of the Study:

  • To develop a microfluidic method for controlled one-to-one encapsulation of particles as cell models.
  • To demonstrate the utility of this method for potential applications in single-cell analysis and cell manipulation.

Main Methods:

  • Utilized an alternating droplet generation encapsulation technique.
  • Employed a microchannel with locally treated wettability for uniform droplet formation.
  • Incorporated spontaneous droplet coalescence using specific surfactants for particle enclosure.

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Title Cell Encapsulation by Droplets
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Title Cell Encapsulation by Droplets

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A Pipette-Tip Based Method for Seeding Cells to Droplet Microfluidic Platforms
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A Pipette-Tip Based Method for Seeding Cells to Droplet Microfluidic Platforms

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Related Experiment Videos

Last Updated: Mar 31, 2026

High Throughput Single-cell and Multiple-cell Micro-encapsulation
16:19

High Throughput Single-cell and Multiple-cell Micro-encapsulation

Published on: June 15, 2012

19.3K
Title Cell Encapsulation by Droplets
13:10

Title Cell Encapsulation by Droplets

Published on: October 1, 2007

9.1K
A Pipette-Tip Based Method for Seeding Cells to Droplet Microfluidic Platforms
06:50

A Pipette-Tip Based Method for Seeding Cells to Droplet Microfluidic Platforms

Published on: February 11, 2019

12.1K

Main Results:

  • Successfully encapsulated fluorescent particles as cell models in uniform water-in-oil-in-water (W/O/W) emulsion droplets.
  • Achieved up to 20% of particle-containing droplets with single-particle encapsulation.
  • Demonstrated successful enclosure of particles within inner aqueous droplets via droplet coalescence.

Conclusions:

  • The alternating droplet generation method enables precise one-to-one encapsulation.
  • This technique offers a versatile platform for applications like high-throughput single-cell assays, gene transfection, and cell fusion.