Antiangiogenic and antiproliferative effects of black pomegranate peel extract on melanoma cell line
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Published on: July 5, 2017
N Dana1, Sh Haghjooy Javanmard1, L Rafiee1
1Applied Physiology Research Center, Isfahan University of Medical Sciences, Isfahan, I.R. Iran.
Abstract:
In the present study possible effects of black pomegranate peel extract (PPE) on the B16F10 melanoma cells proliferation and Human Umbilical Vein Endothelial Cells (HUVECs) angiogenesis were investigated. PPE was added into the cell lines (B16F10 and HUVECs) media with different concentrations (10-450 μg/ml). After 48 h, the cell survival was measured by 3-(Dimethylthiazol-2-yl)-2, 5-diphenyl-tetrazolium bromide (MTT) assay. Angiogenesis was investigated by matrigel assay (PPE (200, 300, 400 μg/ml)); HUVECs, vascular endothelial growth factor (VEGF) mRNA expression was detected by quantitative reverse transcriptase-polymerase chain reaction (QRT-PCR) assay. VEGF concentration in culture medium of HUVECs was determined by enzyme-linked immunosorbent assay (ELISA). PPE had positive anti proliferative effect on melanoma cells in a dose-dependent manner, but not on HUVECs. The matrigel assay results indicated that PPE significantly inhibited length, size and junction of the tube like structures (P<0.05). VEGF mRNA expression and concentration levels in culture medium of PPE treated HUVECs reduced significantly in a concentration-dependent manner (P<0.05). Simultaneous inhibition of melanoma cell proliferation and angiogenesis proposed that, PPE can be a good candidate against melanoma development. Based on the results, PPE could effectively suppress angiogenesis potentially through a VEGF dependent mechanism. Further studies are needed to confirm these results.
Insights
Black pomegranate peel extract (PPE) effectively inhibits melanoma cell growth and angiogenesis. This natural compound shows promise as a potential therapeutic agent against melanoma development by targeting vascular endothelial growth factor (VEGF).
Area of Science:
- Oncology
- Cell Biology
- Natural Product Chemistry
Background:
- Melanoma is an aggressive skin cancer with a high mortality rate.
- Angiogenesis, the formation of new blood vessels, is crucial for tumor growth and metastasis.
- Natural compounds are being explored for their therapeutic potential in cancer treatment.
Purpose of the Study:
- To investigate the anti-proliferative effects of black pomegranate peel extract (PPE) on B16F10 melanoma cells.
- To evaluate the anti-angiogenic properties of PPE on Human Umbilical Vein Endothelial Cells (HUVECs).
- To explore the potential of PPE as a therapeutic candidate for melanoma.
Main Methods:
- Cell viability was assessed using the MTT assay.
- Angiogenesis was studied using a matrigel assay.
- Vascular Endothelial Growth Factor (VEGF) mRNA expression and concentration were measured using QRT-PCR and ELISA, respectively.
Main Results:
- PPE demonstrated a dose-dependent inhibition of B16F10 melanoma cell proliferation.
- PPE significantly suppressed tube-like structure formation in HUVECs, indicating anti-angiogenic activity.
- PPE treatment led to a significant, concentration-dependent reduction in VEGF mRNA expression and protein levels.
Conclusions:
- Black pomegranate peel extract exhibits both anti-proliferative effects on melanoma cells and anti-angiogenic properties.
- PPE may suppress angiogenesis through a VEGF-dependent mechanism.
- PPE holds potential as a therapeutic agent for melanoma treatment, warranting further investigation.
